Eatablishment of rapid diagnosis and prevention of viral infectious disease prevalent in schools

我们发展了手足口病并发呼吸道感染的快速诊断，如腺病毒相关的游泳池热，这是一个学校卫生问题，肠病毒71 （EV71）相关的中枢神经感染，并对诺如病毒感染和预防进行了2年的调查。我们证实了PCR系统对于快速鉴定腺病毒是非常有用的。游泳池介导的4型群众性腺病毒感染在日本暂停了10年之后才出现，因此对游泳池水进行了常规快速分析。我们建立了一套PCR系统，便于从临床标本中直接检测EV71。我们发现，在严重中枢神经感染患者的标本中经常可以检测到EV71，包括EV71相关的死亡和后遗症，这在日本的区域流行期间很少见。这有助于早期诊断和早期策略。此外，该PCR检测系统还为脑脊液中EV71基因的检测提供了便利。由于在手足口病存在的情况下，肠病毒可引起脑病等严重中枢神经感染，除了EV71病毒外，柯萨奇A组6型（CA6）病毒也可引起类似症状，提示在检查时也应检出CA6病毒。此外，我们还对初中生风疹知识进行了调查。风疹可以通过疫苗预防，但是，最近初中生的疫苗接种率很低，未来可能会出现先天性风疹综合征。结果表明，他们对风疹和风疹疫苗的认识不足。在小学和初中，群体性急性肠胃炎多发生在冬季。在许多情况下，食品是通过食品服务人员的手被污染的。应制定预防感染的策略，例如彻底洗手。我们建议在休息室使用盥洗便壶。少

We have developed a rapid diagnosis of hand-foot-and-mouth disease complicated by respiratory infection such as adenovirus-related swimming pool fever, which is an issue in school health, and enterovirus 71 (EV71) -related central nervous infection, and have investigated norovirus infection and prevention for 2 years. We confirmed that a PCR system was very useful for rapidly identifying adenovirus. Since swimming pool-mediated mass adenovirus type 4 infection, which developed after a 10-year pause in Japan, the rapid analysis of swimming pool water has been routinely performedWe established a PCR system, which facilitated direct detection of EV71 from clinical specimens. We showed that EV71 could be frequently detected in specimens from patients with severe central nervous infection, including EV71-related deaths and sequelae, which have been rare in Japan, during a regional prevalence. This has facilitated early diagnosis and early strategies. In addition, the PCR system has facilita … More ted detection of EV71 gene from cerebrospinal fluid, in which gene detection is difficult.As enerovirus causing severe central nervous infection such as encephalopathy in the presence of hand-foot-and-mouth disease, not only EV71 but also Coxsackie group A type 6 (CA6) virus caused similar symptoms, suggesting that CA6 virus should also be detected at examination.In addition, we surveyed knowledge about rubella in junior high school students. Rubella can be prevented with vaccine, however, the rate of vaccination has recently been low among junior high school students, and congenital rubella syndrome may develop in the future. It was shown that their knowledge about rubella and rubella vaccine was insufficient.In elementary and junior high schools, mass acute enterogastritis frequently develops in winter. In many cases, food is contaminated via the hands of food service workers. Strategies for preventing infection, such as thoroughly washing the hands, should be established. We proposed that a Washlet chamber pot should be employed in the rest room. Less