The significance of expression of CD1 family by dendritic cells in human sarcoid lesions

为了研究脂质抗原是否是结节病的致病因子之一，我们研究了脂质抗原呈递分子CD1家族在人结节病变树突状细胞中的表达。以下抗体用于免疫组织化学研究：抗cd1a (O10)，抗cd1b (4A7.6.5)，抗cd1c （L161）和抗cd83 （IM2069）。对8例结节病患者取活检标本，其中皮肤病变1例，淋巴结病变2例，肺病变1例，肌肉病变4例。CD1蛋白在4例瘤样病变的树突状细胞中呈阳性表达。阳性细胞分布在肉芽肿内部和肉芽肿周围。CD1阳性细胞与疾病活动无相关性。此外，在B57BL/6小鼠实验模型中，给药6,6′-二mycolate后，肉芽肿形成与肉瘤样病变相适应。这些结果表明，CD1系统可能在肉瘤病变的发展中起重要作用。

To study whether or not lipid antigens are one of causative agents of sarcoidosis, we investigated the expression of the CD1 family, lipid antigen-presenting molecules, by dendritic cells in human sarcoid lesions.The following antibodies were used for immunohistochemical studies : anti-CD1a(O10), anti-CD1b(4A7.6.5), anti-CD1c(L161), and anti-CD83(IM2069). Biopsied specimens were obtained from 8 patients with sarcoidosis : skin lesion from 1 patient, lymph nodes from 2 patients, pulmonary lesion from 1 patient, and muscle lesions from 4 patients. CD1 proteins were positively expressed by dendritic cells in sarcoid lesions from 4 patients. These positive cells were distributed inside granulomas as well as around granulomas. There were no relations between the cases with CD1 positive cells and their disease activities. In addition, the experimental models on B57BL/6 mice showed granuloma formation that were compatible to sarcoid lesions by the administration of trechalose-6, 6'-dimycolate.These results indicate that the CD1 system may play an important role in the development of sarcoid lesions.