The screaning of endothelial function of young adult patients with Kawasaki disease history

目的：川崎病（Kawasaki disease， KD）被认为是动脉粥样硬化早期的危险因素，因为动脉粥样硬化被认为是一种炎症后疾病。然而，有临床证据证明了这一假设。为了澄清上述问题，有必要建立一种简便且可重复的筛选方法。因此，我们评估了%FMD、PWV、ADMA+SDMA/L-ARG和NOX （NO2- + NO3-）作为有KD病史的年轻成人患者内皮功能和NO相关因素。方法：(1)采用高分辨率超声检测静息时肱动脉内径和反应性充血期间%FD， (2) PWV：采用PWN/ABI (Kolin Inc, Japan),(3)血浆ADMA、SDMA和L-ARG：采用HPLC， (4) NOX：采用毛细管电泳（CE）。结果与讨论：(1)(2)冠状动脉病变（CAL）患者FMD %和PWV均显著升高。两者之间无显著相关。我们怀疑%口蹄疫比PWV更敏感，尽管PWV比%口蹄疫更容易检测，重现性也更好。(3)荧光标记法在特定条件下可检测血浆ADMA、SDMA和L-ARG，而在常规条件下可检测血浆ADMA、SDMA和L-ARG。(4)血浆或尿液NOX：在185nm下，使用基于CE的OFM缓冲液可以检测NOX。不幸的是，在我们的计划中，我们无法获得足够的关于KD患者的数据，因为我们花费了太多的时间来建立这些生化因素值得信赖的检测条件。我们仍在继续这项研究，并计划在不久的将来公布详细的结果

Objects: It is suspected that Kawasaki disease (KD) is a risk factor pre-mature of atherosclerosis, because atherosclerosis is regarded as a post-inflammatory disease. However, there is clinical evidence of the hypothesis. To clarify the above, it is necessary to establish an easy and reproducible screening. Therefore, we evaluated %FMD, PWV, ADMA+SDMA/L-ARG and NOX (NO2- + NO3-) as endothelial function and NO related factors in young adult patients with KD history. Method: (1) %FD: the diameter of brachial arteries at rest and durion reactive hyperemia was measured using high-resolution ultrasound, (2) PWV: using from PWN/ABI (Kolin Inc, Japan), (3) plasma ADMA, SDMA and L-ARG: using HPLC, (4) NOX: using capillary electrophoresis (CE). Results and Discussion: (1) (2) both %FMD and PWV were significantly elevated in the patients with coronary arterial lesions (CAL). There was no significant correlation between both of them. It was suspected that %FMD is more sensitive than PWV, though PWV was rather easier and more reproducible than %FMD. (3) Plasma ADMA, SDMA and L-ARG could be detected under the specific condition for RF-HPLC using fluoreccence label, but under an ordinary condition for UV detection. (4) Plasma or urine NOX: NOX could be detected using OFM buffer based CE at 185nm. Unfortunately, we could not get enough data about KD patients in our schedule, because we spent too much time to establish the trustworthy detecting-condition for these biochemical factors. We still proceed this study, and plan to present the detail results in near future