Development of Bio Sensor Chip Assay

我们开发了一种新的系统来预测癌症患者的疾病结局，利用生物传感器芯片分析。利用表面等离子体共振技术进行生物分子相互作用分析。为了克服传统端粒酶测定（TRAP：端粒重复扩增协议）与PCR伪影相关的缺点和繁琐的PCR后程序，我们开发了一种端粒重复延伸（TRE）测定方法，通过使用表面等离子体共振的生物传感器技术直接测量端粒酶活性作为端粒延伸率。该方法是一种快速、灵敏的方法，而且可以对从体细胞到癌细胞的各种人体细胞进行精确的定量比较。由于其重复性、快速性和简单性，TRE测定适合于端粒酶治疗的临床前和临床试验中评估端粒酶活性的实际应用。

We developed a new system for predicting disease outcome of cancer patients, by using Bio Sensor Chip assay. The assay utilized the surface plasmon resonance technology for the analysis Biomolecular interaction analysis. To overcome the disadvantages of the conventional telomerase assay (TRAP : telomeric repeat amplification protocol) related to PCR artifacts and troublesome post-PCR procedures, we have developed a telomeric repeat elongation (TRE) assay which directly measures telomerase activity as the telomeric elongation rate by biosensor technology using surface plasmon resonance. The assay is rapid and sensitive method, and moreover, allows precise quantitative comparison of a wide range of human cells from somatic cells to carcinoma cells. TRE assay is suitable for practical use in the assessment of telomerase activity in preclinical and clinical trials of telomerase-based therapies, because of its reproducibility, rapidity and simplicity.