The role of tissue factor on lung cancer metastasis

目的;组织因子（Tissue factor， TF）是外源性凝血级联的启动物，在多种癌细胞中也有表达，在独立于凝血途径的癌症进展和转移过程中发挥重要作用。例如，作为一种粘附分子，TF可能在非小细胞肺癌（NSCLC）的转移过程和血管生成中发挥关键作用。方法：为了进一步研究TF在非小细胞肺癌肿瘤细胞侵袭中的作用，我们在LightCycIer中使用实时定量逆转录聚合酶链反应检测了42例非小细胞肺癌患者肿瘤中TF mRNA的表达。然后，我们将TF mRNA的表达与肿瘤细胞侵袭血液和淋巴管的组织学证据进行比较。结果：虽然TF mRNA的表达与淋巴管浸润无显著相关性，但浸润血管的肿瘤中TF mRNA的表达量（Log_<10 >）显著高于未浸润血管的肿瘤（1.59±0.16,P = 0.03） （TF mRNA/GAPDH mRNA = 2.16±0.18）。结论：提示TF在非小细胞肺癌肿瘤细胞侵袭血管中起重要作用。

Purpose; Tissue factor (TF), an initiator of the extrinsic coagulation cascade, is also expressed in a wide range of cancer cells and plays important roles in cancer progression, and metastasis, processes independent of the blood coagulation pathway. For example, by acting as an adhesion molecule enabling tissue invasion, TF may play a key role in the metastatic process and angiogenesis in non-small cell lung cancer (NSCLC). Methods: To further investigate the role of TF on tumor cell invasion in NSCLC, we measured TF mRNA expression in the tumors of 42 NSCLC patients using real time quantitative reverse transcription polymerase chain reaction carried out in a LightCycIer. We then compared TF mRNA expression with the histological evidence of invasion of blood and lymphatic vessels by tumor cells. Results: Although, there was no significant relationship between TF mRNA expression and invasion of lymphatic vessels, TF mRNA expression was significantly higher in tumors that invaded blood vessels (Log_<10 >TF mRNA/GAPDH mRNA = 2.16 ± 0.18) than in those that did not (1.59 ± 0.16, P = 0.03). Conclusion: These results suggest that TF plays a major role in blood vessel invasion by tumor cells in NSCLC.