Experimental Study on skeletal Tissue allotransplant

异基因器官移植后的嵌合体可能是诱导供者特异性移植物接受的一种机制。然而，对同种异体骨组织移植后移植物细胞的迁移知之甚少。我们的研究调查了同种异体肢体移植后的细胞交换现象。实验动物为DA大鼠和Lewis大鼠，右后肢成对原位移植。移植后给予FK506(藤泽制药公司，日本大阪)进行免疫抑制治疗。用半定量聚合酶链式反应检测Y染色体的嵌合性。结果表明：1)Y染色体在同种异体肢体移植中并不具有男性特异性次要抗原的功能；2)移植肢体功能较健侧恢复约70%；3)供体细胞在移植后1年内存活，并逐渐迁移到受者的淋巴组织和骨骼中；4)供体细胞在移植肢体(包括骨、肌肉和皮肤组织)中逐渐被受体细胞取代。我们的研究显示了同种异体肢体移植后稳定和高水平的嵌合体。

Chimerism following allogeneic organ transplantation may be a mechanism for induction of donor-specific graft acceptance. However, little is known about graft cell migration following skeletal tissue allograft. Our study investigated a cell exchange phenomenon following whole-limb allotransplantation. Experimental animals were DA rats and Lewis rats and right hind-limbs were orthotopically transplanted in sex-mismatched pairs. FK506 (Fujisawa Pharmaceutical Co., Osaka, Japan) was administered after transplantation for immunosuppression. Chimerism was assessed by semi-quantitative polymerase chain reaction for the Y-chromosome. Our results indicated that 1) Y-chromosome didn't behave as male specific minor antigen in whole limb allograft, although it worked as antigen in skin grafts 2) function of transplanted limb was roughly recovered 70% compared to normal side, 3) donor cells survived in the graft through 1 year after transplantation and gradually migrated into recipient's lymphoid tissue and bones, 4) donor cells in the grafted limb (involving bone, muscle and skin tissues) were gradually replaced by recipient's cells. Our study demonstrated stable and high level chimerism following limb allotransplantation.