Experimental Study on skeletal Tissue allotransplant

异基因器官移植后的嵌合体可能是诱导供体特异性移植物接受的机制。然而，很少有人知道移植后的骨组织移植细胞迁移。我们的研究调查了全肢同种异体移植后的细胞交换现象。实验动物为DA大鼠和刘易斯大鼠，右后肢以性别不匹配的方式原位移植。FK 506（Fujisawa Pharmaceutical Co.，Osaka，Japan）用于免疫抑制。通过半定量聚合酶链反应评估Y染色体的嵌合体。结果表明：（1）Y染色体在全肢移植中不作为男性特异性次要抗原，但在皮肤移植中作为抗原发挥作用;（2）移植肢体功能恢复较健侧约70%;（3）供者细胞在移植物中存活至移植后1年，并逐渐迁移至受者的淋巴组织和骨组织中;（4）移植肢体（包括骨、肌肉和皮肤组织）中的供体细胞逐渐被受体细胞所取代。我们的研究表明，稳定和高水平的嵌合体后，异体肢体移植。

Chimerism following allogeneic organ transplantation may be a mechanism for induction of donor-specific graft acceptance. However, little is known about graft cell migration following skeletal tissue allograft. Our study investigated a cell exchange phenomenon following whole-limb allotransplantation. Experimental animals were DA rats and Lewis rats and right hind-limbs were orthotopically transplanted in sex-mismatched pairs. FK506 (Fujisawa Pharmaceutical Co., Osaka, Japan) was administered after transplantation for immunosuppression. Chimerism was assessed by semi-quantitative polymerase chain reaction for the Y-chromosome. Our results indicated that 1) Y-chromosome didn't behave as male specific minor antigen in whole limb allograft, although it worked as antigen in skin grafts 2) function of transplanted limb was roughly recovered 70% compared to normal side, 3) donor cells survived in the graft through 1 year after transplantation and gradually migrated into recipient's lymphoid tissue and bones, 4) donor cells in the grafted limb (involving bone, muscle and skin tissues) were gradually replaced by recipient's cells. Our study demonstrated stable and high level chimerism following limb allotransplantation.