The relationship of the inflammatory infiltrating cell in prostate grand and chemokine

(1)前列腺炎性细胞浸润的病理学研究1998~2000年，因浸润性膀胱癌而行根治性膀胱癌切除术的患者。采用CD2O、CD45RO、CD68、S-100等一系列抗体进行免疫组织化学染色。浸润性细胞以T细胞为主。(2)抗菌药物及抑制细胞因子分泌。以人前列腺癌PC-3细胞为实验对象。上清液中IL-8的浓度随人支原体浓度的增加而升高。RT-PCR检测到PC-3细胞中有Toll样受体-2和Toll样受体4的mRNA表达。随着人型支原体浓度的增加，NF-κB活性增加。这些数据表明，Toll样受体的信号上调了NF-κB的活性，并导致IL-8的产生。RT-PCR法检测GFLX对IL-8mRNA表达的影响。Gflx可显著降低肿瘤坏死因子-α诱导的IL-8mRNA水平。GFLX以剂量依赖的方式抑制PC-3分泌IL-8。提示GFLX对慢性前列腺炎有抗炎作用。

(1)The pathological study of inflammatory cell infiltration in the prostate in patientsPatients entered into the study received radical cystoprostatectomy because of invasive bladder cancer in our institute from 1998 to 2000. The infiltrating cell type was assessed by immunostaining using a series of antibodies CD2O, CD45RO, CD68,S-100. The types of infiltrating cells were dominantly T cells. Inflammatory cell infiltration was seen in 32.4% cases.(2)Antimicrobial agents and suppression of cytokine secretion of prostate cancer cell line.PC-3,a human prostate cancer cell lines, was used for the experiment. IL-8 concentrations of the supematants were increased depending on the concentrations of Mycoplasma hominis. The mRNA of Toll like receptor-2 and Toll like receptor4 were demonstrated by RT-PCR in PC-3 cell lines. The activities of NF-κB were increased depending on the concentration of Mycoplasma hominisa. These data show that the signals from Toll-like receptor up the activities of NF-κB and lead to produce of IL-8. The influence of GFLX on IL-8 mRNA expression was analyzed using RT-PCR. GFLX significantly attenuated the TNF-α induced IL-8 mRNA level. GFLX suppresses the secretion of IL-8 from PC-3 in a dose-dependent manner. The results may imply that GFLX has an anti-inflammatory effect on chronic prostatitis.