Role of leptin and interleukin-8 in human ovary

我们评估了瘦素和白细胞介素（IL-8）在人卵巢中的表达、调节和作用。在IVF周期中收集卵泡液和颗粒黄体细胞（GLC）。还使用了人颗粒细胞肿瘤细胞系KGN细胞。采用ELISA法检测卵泡液中瘦素、IL-8水平及IL-8蛋白的产生。卵泡液中瘦素水平与IL-8水平呈正相关。逆转录聚合酶链反应显示GLC和KGN细胞中存在瘦素受体mRNA和IL-8 mRNA。相反，IL-8受体mRNA在这些细胞中未检测到。E2和FSH的加入显著增加了KGN细胞IL-8蛋白的产生，但对IL-8蛋白的产生没有影响。在GLC中。另一方面，KGN细胞和GCL中IL-8蛋白的产生不受瘦素的影响。GLC和KGN细胞中IL-8的表达通过加入IL-1 β和TNF α而增加。IL-8促进卵巢基质细胞增殖。IL-1 β可诱导KGN细胞pIkappaB表达，APDC可抑制IL-1 β诱导的KGN细胞pIkappaB表达。IL-1 β通过激活颗粒细胞NF-κ B诱导的IL-8可能在卵泡成熟的排卵期起作用。

We evaluated the expression, regulation, and role of leptin and interleukin (IL-8) in human ovary. Follicular fluid and granulosa lutein cells (GLCs) were collected during IVF cycles. KGN cells, the human granulosa cell tumor cell line, were also used. The levels of leptin and IL-8 in follicular fluid and IL-8 protein production were determined using ELISA. The levels of leptin in follicular fluid showed a positive correlation with those of IL-8. Reverse transcription polymerase chain reaction showed the presence of leptin receptor mRNA and IL-8 mRNA in GLCs and KGN cells. In contrast, IL-8 receptor mRNA was not detected in these cells. The addition of E2 and FSH significantly increased the IL-8 protein production in KGN cells, whereas there were no effects on the IL-8 protein production. in GLCs. On the other hand, IL-8 protein production was not influenced by leptin in KGN cells and GCLs. The expression of IL-8 in GLCs and KGN cells was increased by addition of IL-1beta and TNFalpha. Interleukin-8 increased the proliferation of ovarian stromal cells. The expression of pIkappaB in KGN cells was induced by IL-1beta, and the effects were reduced by APDC. Interleukin 8 induced by IL-1beta via activation of NF-kappaB in granulosa cells may have a role in the periovulatory period of follicular maturation.