EMBRYO-DERIVED PLATELET-ACTIVATING FACTOR MODULATES ENDOMETRIAL AND DECIDUAL EXPRESSION OF ADHESION MOLECULES DURING IMPLANTATION.

血小板活化因子（PAF）参与了包括着床在内的许多生殖过程。为了研究PAF在着床中的作用，我们评估了子宫内膜基质细胞（ESC）和蜕膜细胞（DC）中各种粘附分子的表达。我们用类似物carbamyl-PAF （C-PAF）刺激后，测量了ESC和DC中三种粘附分子的表达。这种底物不被代谢。这三种粘附分子分别是整合素α1、整合素β1和纤维连接蛋白。对这些整合素进行免疫生化分析，对纤维连接蛋白进行酶联免疫吸附试验。未受刺激的ESC组织均弱表达整合素α1和β1。C-PAF刺激后，这些粘附分子在ESC和DC中的表达增加。c - paf对DC的刺激比ESC更明显。C-PAF以剂量依赖的方式增加ESC和DC中纤维连接蛋白的产生。在用PAF受体拮抗剂Y-24180和CV2086预处理后，这些c -PAF诱导的效应被消除。这表明，蜕膜组织和发育中的胚胎分泌的PAF刺激了这些粘附分子在母胎界面的表达。

Platelet-activating factor (PAF) has been implicated in a number of reproductive processes including implantation. To investigate the role of PAF in implantation, we evaluated the expression of various adhesion molecules in endometrial stromal cells (ESC) or decidual cells (DC). We measured the expression of three adhesion molecules in ESC and DC following stimulation with an analogue, carbamyl-PAF (C-PAF). This substrate is not metabolized. These three adhesion molecules are integrin α1, integrin β1, and fibronectin. Immunohystochemical analysis for these integlins and an enzyme-linked immunosorbent assay for fibronectin were used. The non-stimulated ESC constitutively expressed both integrin α1 and β1 weakly. The expression of these adhesion molecules in ESC and DC was increased by the stimulation with C-PAF. The C-PAF-induced stimulation in DC was more evident than that in ESC. C-PAF increased the production of fibronectin in ESC and DC in a dose-dependent manner. These C-PAF-induced effects were abolished following pretreatment with PAF receptor antagonists, Y-24180 and CV2086. It is suggested that the PAF secreted by the decidual tissue and by the developing embryo stimulates the expression of these adhesion molecules at the maternal-fetal interface.