Basic Research for Allograft of Omentum

显微外科重建使用网膜瓣最近取得了显著进展。然而，我们不能使用网膜瓣的情况下使用或切除。所以同种异体网膜瓣移植是有用的。本文报道了网膜异体的基本研究。首先，重要的是知道大网膜缺血时间的容忍极限。因此，我们提出了一项实验研究，在该研究中，我们检查了不同持续时间的止血后网膜的状况。以成年Wistar大鼠胃网膜血管为基础，大网膜瓣升高。为建立缺血时限标准，在1 ~ 6 h内每小时制作大网膜缺血模型。10分钟后，在宏观和微观下检查网膜瓣。分别于去钳后10分钟、1天、2天、3天、7天观察2小时缺血大网膜模型的恢复过程，建立评价大网膜恢复的形态学标准。2小时缺血大网膜模型与正常大网膜大体相似。缺血网膜时限估计为3 ~ 4小时。去断路3天后，2小时缺血模型组织学标本显示成纤维细胞浸润和血管形成。脱支7天后2小时模型组织学标本显示部分胶原纤维重构。第二步，由于同种异体移植使用免疫抑制剂，研究了裸鼠网膜瓣的伤口愈合情况。有大网膜覆盖的创面在正常皮肤中基本愈合，无大网膜的创面愈合如疤痕。网膜瓣对裸鼠创面愈合有一定的促进作用。我们的研究是同种异体网膜移植的第一步。

Microsurgical reconstruction using the omental flap has recently made marked progress. However, we cannot use the omental flap in cases which it was used or resected before. So allograft of the omental flap will be useful. We report the basic research for the allograte of omentum. At first, what is of great importance knows the tolerance limits for omental ischemic time. For this reason, we present an experimental study in which we examined the condition of the omentum after hemostasis of various durations. The omental flaps were elevated based on gastroepiploic vessels in adult Wistar rats. Ischemic omental models were made hourly from 1 to 6 hours in order to establish a standard for ischemic time limit. After 10 minutes, the omental flaps were examined macroscopically as well as microscopically. The recovery process of 2-hour ischemic omental models was examined at 10 minutes, 1 day, 2 days, 3 days, and 7 days after declamping, to establish a morphological standard for the evaluation of omental recovery. Ischemic omental models of 2 hours were similar to normal omentum macroscopically and microscopically. The ischemic omental time limit was estimated to range from 3 to 4 hours. A histological specimen of 2-hour ischemic model examined 3 days after declamping showed vascularization and infiltration of fibroblasts. A histological specimen of 2-hour model examined 7 days after declamping showed remodeled collagen fiber partially. At second step, wound healing using the omental flap in nude mouse was examined, because immunosuppressant is used at allograft. The wound covered with omentum was almost healed in normal skin and the wound without omentum was healed such as a scar. The omental flap was useful for wound healing in nude mouse. Our research was a first step for the allograft of omentum.