Pathogen enzyme productivity of bacteria isolated from a therapy resistant periapical periodontitis cases

难治性根尖周炎是由于粪肠球菌、白色念珠菌、铜绿假单胞菌和口腔链球菌等抵抗牙髓治疗的细菌持续感染而引起的。难治性根尖周炎多为亚急性病例，但症状多样。本研究明确了耐药菌对病原菌酶活性的影响，并探讨了耐药菌对难治性根尖周炎临床表现的影响。型菌株（ATCC35039）和试验菌株口腔链球菌不表现出产酶特征，粪链球菌表现出产软骨骨素硫酸酯酶，铜绿假单胞菌表现出产卵磷脂酶和产胶原酶、透明质酸酶、软骨骨素硫酸酯酶等弱酶的频率较高。结果表明，这些细菌的病原菌产酶特性参与了难治性根尖周炎临床症状持续发展的可能性。

Refractory periapical periodontitis is caused by persistent infection by bacteria to resist for endodontic treatment such as Enterococcs faecalis, Candida albicans, Pseudomonas aeruginosa and Streptococcus oralis.Many of refractory periapical periodontitis cases are subacute cases, but those symptom is various.The bacteria which resisted therapy examined productivity in pathogen enzyme for the purpose of it was clear, and in this study, these doing the effect which therapy resistant bacteria gave to clinical manifestation expression of a refractory periapical periodontitis case.The type strain (ATCC35039) and the test strain of S.oralis did hot show pathogen enzyme production characteristics.E.faecalis showed chondoroitin sulfatase producing.P.aeruginosa showed lecithinase producing and weak producing such as collagenase, hyaluronidase and chondoroitin sulfatase in high frequency.These resultant showed the probability which pathogen enzyme production characteristics of these bacteria participated in refractory periapical periodontitis developing for clinical symptom persistently.