The effect of thermochemotherapy on cervical lymph node metastasis

（1）引言本实验研究了热化疗对金黄地鼠口腔鳞癌（O-1 N）原发灶和颈淋巴结转移的影响。（2）材料与方法将O-1 N移植于金黄地鼠颊囊内的动物分为4组：高温组（HG），每周2次，每次43℃，每次40分钟;化疗组（CG），每周2次，每次10 mg/kg，腹腔注射多西他赛;热化疗组（TCG）在热疗前即刻给予多西他赛，未治疗组（UG）。当颊部肿瘤直径达到7 mm时，用13.56MHz射频电容热疗系统对其进行加热。采用C-C热电偶温度计连续测量瘤内温度。治疗后3周和4周分别计算肿瘤体积，切除原发肿瘤和颈部淋巴结，并进行组织学检查。（3）结果治疗后，对照组肿瘤体积明显缩小，高、中剂量组肿瘤体积明显缩小。组织学证实CG组19只动物中0只（0%）、HG组16只动物中3只（18.5%）和TCG组15只动物中11只（73.3%）的肿瘤细胞完全消失。CG组淋巴结转移率为42.1%（8/19只动物），HG组为6.3%（1/16只动物），TCG组为13.3%（2/15只动物），而UG组为88.9%（8/9只动物）。所有组的淋巴结转移的组织学模式均在淋巴结包膜内得到证实。热疗联合多西他赛化疗可增强抗肿瘤作用，热疗和热化疗均能抑制淋巴结转移。

(1).IntroductionThe effect of thermochemotherapy on hamster oral squamouscell carcinomas (O-1N) were studied in their primaiy sites and in their cervical lymph node metastasis.(2).Materials and MethodsGolden hamster in which O-1N was transplanted into the cheek pouch were divided into 4 group: a hyperthermia group(HG) subjected to radiofrequency(RF) hypothermia at 43℃ for 40 minutes twice a week, a chemotherapy group(CG) in which the docetaxel was administered twice a week (10mg/kg i.p.), a thermochemotherapy group(TCG) in which docetaxel was given immediately before hyperthermia, and an untreated group(UG). When the buccal tumor reached 7mm in diameter, it was heated with a 13.56 MHz RF capacitive hyperthermia system. Intratumoral temperature was measured continuously by the C-C thermocouple thermometer. The tumor volume was calculated, primary tumor and cervical lymph nodes were excised three and four weeks after treatment, respectively, and were examined histologically.(3).ResultsThe primary tumor size was inhibited after treatment in the CG, it was decreased in the HG and TCG. Complete disappearance of tumor cells was confirmed histologically in 0 of 19 animals (0%) in the CG,3 of 16 animals (18.5%) in the HG,and 11 of 15 animals (73.3%) in the TCG. The incidence of lymph node metastasis was 42.1%(8 of 19 animals) in the CG,6.3%(1 of 16 animals) in the HG,and 13.3% (2 of 15 animals) in the TCG, compared with 88.9% (8 of 9 animals) in the UG. The histological pattern of lymph node metasitasis in the all group were confirmed within the lymph node capsule. In conclusion, the anti-tumor effect was enhanced when hyperthermia was combined with chemotherapy by docetaxel, both hyperthermia and thermochemotherapy inhibited lymph node metastasis.