Cell-biological study for Biological production of Proteoglycans in the Articular-disc in the case of Internalderangement Temporomandibular Joint

许多患者因颞下颌关节（TMJ）内部紊乱而出现疼痛和紊乱，但病因尚不清楚。首先，我在德国蒂宾根大学解剖研究所进行研究，在完全内脱位变形的关节盘中，在椎间盘的前下段发现了高浓度的硫酸角蛋白和硫酸软骨素-6，并发现滑膜增生。在临床的内部紊乱病例中，Tenascin m-RNA在增生的滑膜中被识别，这意味着发生了重塑和繁殖。在这种情况下，COX-1和COX-2分布在增生滑膜的血管附近。我们还从内部紊乱的TMJ病例中建立了滑膜细胞系，在缺氧和白细胞介素-1b的刺激下，细胞通过RT-PCR自分泌MMP-1、MMP-3、MMP-9和MMP-13，但随着培养细胞的上膜，ELISA检测发现滑膜细胞中硫酸角蛋白浓度降低。

Many patients who are suffering from pain and disorder due to internalderangement on temporomandibular joint (TMJ) visited our clinic but the etiology still unclear. At first, in Anatomy institute, Tubingen university Germany, I researched, in the completely internalderangement deformed articular disc, high concentration of keratan-sulfate and chondroitin-6 sulfate were identified in the antero-inferior part of the disc and hyperplastic synovial membrane was identified in these cases. In the clinical internalderangement cases, Tenascin m-RNA was recognized in the hyperplastic synovial membrane which means remodeling and reproduction were occurred. In such cases, COX-1 and COX-2 were distributed near the vascular vessels in the hyperplastic synovial membrane.We also established the synovial cell line from internalderangement TMJ cases, with the stimulation of hypoxia and Inter-leukin-1b, the cell line autocrined MMP-1, MMP-3, MMP-9 and MMP-13 by RT-PCR but with the supernnat of the culture cells, there was the decrease of concentration of keratan sulfate in the synovial cells by ELISA.