Pathologic Studies on Induction of Craniomandibular Joint Arthritis is using in Rabbits Escherichia Coli Antigens

材料与方法：日本雄性家兔用大肠杆菌0：14和弗氏不完全佐剂致敏。对照组用生理盐水灌流，用纯净水灌流。实验组用生理盐水灌流左颞下颌关节（TMJ）下腔，再灌注大肠杆菌0：14和纯净水。术后7<th>、14、<th>21、28<th>、35<th>、56<th>天分别处死动物，切除关节组织进行病理学观察。在Stainblocker、Larsen、亚瑟和Amaji等分类法的基础上，我们提出了一种新的分类方法，并用评分法进行比较。整个期间的评分为0级。（实验组）：第7<th>天：观察到急性渗出性炎症。第14<th>天：由于血管翳增殖，载物被破坏。第21天：病变发展到家中，这表明向慢性炎症过渡。第28<th>天：进一步病变发展。可见典型类风湿性关节炎，巨噬细胞、浆细胞、淋巴细胞融合滤泡或血管翳形成。在这之后，它看起来有好转，逐渐得到治愈。现在我们对MMP-3和obteopontin进行随访，在评分方面，关节炎破坏如前所述得到促进，在28天达到最高点<th>，（3.00+/-0.00）.备注：由于实验组的病理过程与人类颞下颌关节的病理过程相同，本实验不仅为阐明类风湿性关节炎的实际形态及今后的治疗研究提供了依据。

Material and Method : While Japanese male rabbits were sensitized by Escherichia coli 0 : 14 and Freunds incomplete adjuvant. For the control group, rabbits were perfused with physiolosical saline and them purifyied water. On the other hand, for the experimental group, their lower cavity of the left temporomandibular joint (TMJ) were perfused with physiolosical saline and then infused with Escherichia coli 0 : 14 together with purifyied water. Several pieces of the latter were sacrificed on the 7^<th>, 14^<th>, 21st, 28^<th>, 35^<th>, and 56^<th> day after the above procedure for pathological observation by resecling the joint. Based on stainblocker's, Larsen's, Arthur's and Amaji's dassification, we invented a new classification method of our own for giving them comparison using scores.Results : (Control group) : No pathological variation was observed at the basic structure, although the carfilage layer got thinner with time. Score was class 0 throughout the entire period. (Experimental group) : 7^<th> day : An acute exudative inflammation was observed. 14^<th> day : The carfilage was destroyed owing to multiplication of pannus. 21st day : The lesion developed to reach the home, which indicates the transition to chronic inflammation. 28^<th> day : Further lesion development. Typical rheumatiod arthritis was observed, macrophage, plasma cell, conflent follicle of limphocyte, or formation of pannus. After this, it look a turn for the better to get cured gradually. Now we are following up about MMP-3 and obteopontin.As to the scores, arthritis destruction was promoted as described above, and it showed maximum point on the day of 28^<th>, which was (3.00+/-0.00).Remark : As the pathological process of the experimental group is just common to that of human TMJ, it is confimied that this experiment serves not only for illuminating the actual shape of rhemaloid arthritis and also the future therapeulic research.