Searches for novel tumor markers for gastrointestinal cancers by ProteinChip technology

众所周知，习惯性饮酒是多种癌症的风险因素。由于个人和口头上对饮酒情况的报告不一定准确，因此需要客观的指标来评估饮酒情况。然而，现有的筛查过量饮酒者的标记物的灵敏度和特异度有限，因此有必要寻找新的标记物。最近，表面增强激光解吸电离飞行时间质谱仪(SELDI-TOF MS)已成功地用于检测复杂生物标本中的疾病相关蛋白。我们使用蛋白质芯片SELDI技术对16例慢性酒精中毒患者戒酒期间连续采集的血清样本进行比较蛋白质谱分析，我们识别出两个峰值(5.9kD和7.8kD)，这两个峰在入院时都被下调，而在戒酒一周后，其表达水平显著上升。这些变化也见于G-谷氨酰基转移酶无反应者。对这两种蛋白进行了部分纯化和氨基酸序列测定。类似的策略也被用于检测肝癌和结肠癌的诊断标志物。

It is well established that habitual drinking is a risk factor for a variety of cancers.Since personal and verbal reporting of alcohol use is not necessarily accurate, objective markers to assess alcohol consumption are required. The currently available markers, however, are limited in sensitivity and specificity for screening of excessive alcohol drinkers, Therefore, searches for novel markers are warranted.Recently, surface-enhanced laser/desorption ionization time-of-flight mass spectrometry (SELDI-TOF MS) has been successfully used to detect disease-associated proteins in complex biological specimens. We used the ProteinChip SELDI technology to generate comparative protein profiles of the consecutive serum samples obtained during abstinence from a total of 16 chronic alcoholic patients hospitalized for a rehabilitation program, We recognized two peaks (5.9 kD and 7.8 kD), both of which had been down regulated on admission, the expression level of which significantly increased after a one-week abstinence. These changes were also seen in non-responders of g-glutamyltransferase. These two proteins were partially purified and subjected to amino acid sequencing. Similar strategy has been applied for detection of diagnostic markers for liver cancers and colon cancers.