The ratio of beta catenin to APC in tumor cell

在癌症的预后诊断中，评估肿瘤的侵袭和转移潜力以及对治疗的反应性是检验医学领域的重要课题。为了评估对放疗或化疗的反应性，我们检查了细胞凋亡途径的完整性。为此，首先分析p53的突变或Bc12的表达，然后分析凋亡通路中各因子的表达。所获得的数据可用于临床实践。为了有效评估其侵袭和转移潜力，分析cadherin- β catenin的表达被认为是一种可能的选择。-连环蛋白是一种多功能蛋白，既是粘附体连接的组成部分，也是信号转导途径的关键成员。β -连环蛋白的表达水平受泛素-蛋白酶体蛋白降解途径的调控。在该系统中，肿瘤抑制基因APC产物参与了β -连环蛋白的降解。在最近的日本癌症协会年会上，讨论了β - catenin-表达与癌细胞状态的关系。在本研究中，我们试图用结肠癌标本检测β - catenin和APC的表达，但没有得到全面的结果。因此，我们总结β -连环蛋白分析在检验医学中对肿瘤诊断的意义如下。1)癌细胞中β -连环蛋白基因突变的检测。2)分析β - catenin的表达水平取决于癌细胞的来源。3) APC等调节β - catenin表达的分子分析。

In the diagnosis of prognosis of cancer, the assessment of the invasive and metastatic potential in addition to reactivity to the therapy is significant subject in the field of laboratory medicine. To assess the reactivity to the treatment of either radiation therapy or chemotherapy, the integrity of apoptosis pathway is examined. In this purpose, mutation of p53 or the expression of Bc12 is analyzed firstly and then expression of each factor in the apoptosis pathway is analyzed. The obtained data are profitabley utilized for the clinical practice.For the effective method to assess the invasive and metastatic potential, analysis of the expression of cadherin-beta catenin is considered as a possible candidate. Beta catenin is a multifunctional protein that is both an integral component of adherens junctions and a pivotal member of a signal transduction pathway. The expression level of beta catenin is regulated by ubiquitin-proteasome protein degradation pathway. In the system, tumor suppressor gene APC product contributes the beta catenin degradation.In recent annual meeting of Japanese Cancer Association, the relation of beta catenin-expression and status of the cancer cells have been discussed. In this study, we attempted to examine the expression of beta catenin and APC using specimens from colon cancer, but did not comprehensive results. Therefore, we summarize the significance of analysis of beta catenin for cancer-diagnosis in laboratory medicine as follows. 1)Detection of the mutation of beta catenin gene in cancer cell. 2)Analysis of the expression level of beta catenin depends on the origin of cancer cell. 3)Analysis of the molecules including APC that regulate the expression of beta catenin.