Decomposition of dyes by Bilirubin Oxidase

用胆红素氧化酶(BO)对10种商品蒽醌染料和8种商品偶氮染料的水溶液进行了处理，考察了酶对印染废水的去克隆作用。通过对加入该酶的染料溶液的可见光谱和紫外吸收光谱的测量，证实了该酶对所有蒽醌染料的有效脱色。将加入该酶的纯化C.I.酸性蓝40的水溶液在40℃下摇动，去除酶后，对规定时间后的溶液进行高效液相色谱分析。色谱图显示，加入该酶后，染料(C.I.酸性蓝40)立即开始分解。保留时间在5.2min左右的峰与分解络合物有关，在加入酶后逐渐增加，在3h左右达到，即使在24 h后也几乎保持不变，但逐渐减少。测定了6种偶氮染料溶液在酶存在下的可见光和TJV吸收光谱，证实了该酶对6种偶氮染料的有效脱色作用，但对C.I.直接黑22和C.I.直接紫48两种偶氮染料的脱色效果不明显。并用高效液相色谱法测定了染料的脱色率。色谱图显示，这8种染料在加入酶后立即开始分解。根据色谱峰面积计算，前六种染料的脱色率约为100%，后两种染料的脱色率约为90%。

In order to examine the decolonzation of dyeing effluent by enzyme, aqueous solutions of ten commercial anthraquinone dyes and eight commercial azo dyes were treated with Bilirubin oxidase (BO). Efficient decolorization ofall anthraquinone dyes was confirmed by measuring the visible and UV absorption spectra of the dye solution to which the enzyme was added.An aqueous solution of purified C.I. Acid Blue 40, to ^hich the enzyme wasadded, was shaken at 40 ℃.Aliquots of the solution after the specified time were analyzed by HPLC after removing the enzyme. Thechromatograms showed that the decomposition of dye (C.I. Acid Blue 40) started immediately after the addition of the enzyme. A peak around a retention time of 5.2 min,which can be related to the decomposition complex, increased gradually after the addition of the enzyme and was reached after about 3 h. That peak was nearly maintained even after 24 h, although it gradually decreased. Theabsorption spectrum of the product at that peak was determined by a photodiode array analysis.Efficient decolorization of six azo dyes was confirmed by measureing the visible and tJV absorption spectraof the dye solution in the presence of the enzyme, but that of the two azo dyes, C.I. Direct Black 22 and C.I.Direct Violet 48 was not efficient. Also HPLC was introduced to measure decolorization of dyes. The chromatograms showed that the decomposition of the eight dyes started immediately after the addition of the enzyme. The decolorization ratios calculated from the peak areas in the chromatograms were ca. 100% for first the six dyes and 90% for that last two dyes.