Transcriptional and post-translational regulation of cell adhesion mediated by the cell adhesion molecule, selectin, on lymphocytes.

传统的非硫酸盐化唾液酸化Lewis x和唾液酸化6-磺酸Lewis x决定簇参与淋巴细胞的细胞黏附。非硫酸化唾液酸化Lewis x的表达主要受岩藻糖基转移酶VII在转录水平上的调控，CREB/ATF家族转录因子以及GATA-3、Sp1和MZF-1参与其转录调控。此外，我们还发现转录因子Thp-1(Gli-2)、HIF-α和HIF-α也在唾液酸化Lewis X的表达调控中发挥重要作用。另一方面，GlcNAcβ：6-O-磺酸转移酶在唾液酸基6-磺基Lewis x表达的调节中起重要作用。在已知的5种GlcNAcβ：6-O-磺基转移酶同工酶中，GlcNAC6ST-1和HEC-GlcNAC6ST与调节淋巴细胞中唾液酸基6-磺基Lewis x的表达密切相关。GlcNAcβ：6-O-磺酸转移酶的产物也在另一种细胞黏附分子CD44介导的细胞黏附调节中发挥重要作用，CD44也被认为参与了淋巴细胞的常规募集。

Conventional non-sulfated sialyl Lewis x and sialyl 6-sulfo Lewis x determinants are involved in the cell adhesion of lymphocytes. Expression of non-sulfated sialyl Lewis x is mainly regulated by fucosyltransferase VII at a transcriptional level, and transcriptional factors belonging to the CREB/ATF family as well as GATA-3, Sp1 and MZF-1 are involved in its transcriptional regulation. In addition, we showed the transcriptional factors THP-1 (Gli-2), HIF-α and HIF-α also play important roles in regulation of sialyl Lewis X expression. On the other hand, GlcNAcβ : 6-O-sulfotransferases figure heavily in the regulation of sialyl 6-sulfo Lewis x expression. Among the five known isoenzymes of GlcNAcβ: 6-O-sulfotransferases, we showed that the isoenzymes called GlcNAC6ST-1 and HEC-GlcNAC6ST are closely involved in the regulation of sialyl 6-sulfo Lewis x expression in lymphocytes. The products of GlcNAcβ: 6-O-sulfotransferases also play significant role in the regulation of cell adhesion mediated by another cell adhesion molecule, CD44, which has also been known to be involved in the routine recruitment of lymphocytes.