THE STUDY FOR REGULATION OF THE DEVELOPMENTAL DYNAMICS OF LYMPHATIC SYSTEMS IN HEAD AND NECK, AND FOR THE ADHESION SYSTEMS OF CANCER CELLS TO LYMPHATIC ENDOTHELIUM

我们已经清楚，人淋巴内皮表达桥粒蛋白，桥粒蛋白是桥粒的一个组成部分。在本研究中，桥粒相关蛋白的表达；对上颌上颌上颚及舌部口腔鳞状细胞癌（OSCC）进行菌斑中黏菌素和血小板红蛋白、核心中黏菌素和黏菌球蛋白的检测。结果显示：1)癌组织淋巴内皮表达桥粒蛋白，2)淋巴管OSCC过表达桥粒蛋白，3)OSCC有桥粒核心丢失的倾向。桥粒蛋白在OSCC细胞系上表达的研究HSC2、HSC3 HSC4、HO1myu1 HO1N1, Ca。结果表明：1)所有细胞系均不表达桥蛋白1和2,2)KB不表达桥蛋白1-3,3)除Ca.922外，所有细胞系均不表达桥蛋白1,4)HSC4、HO1myu1、HO1N1，SAS很少表达桥蛋白2,5)所有细胞系均表达桥蛋白3、血小板红蛋白和桥蛋白1和2,6)HO1myu1、HO1N1、Ca。922和SAS中，桥蛋白在细胞间接触表达，但在HSC2、HSC3和HSC4中，桥蛋白在细胞内扩散。OSCC可能与淋巴管结合，表面暴露的桥蛋白在细胞上过表达。此外，在p53突变的细胞系和组织中，OSCC细胞也在细胞内过表达桥蛋白。desmoplakin的过度表达可能与p53突变有关。人新生儿淋巴内皮细胞（HNDLEC）的细胞生长试验表明，在缺乏血管内皮生长因子（VEGF）和胰岛素样生长因子（IGF）的情况下，HNDLEC不能增殖。HNDLEC与HSC2、HSC3、HSC4、HO1myul、HO1N1、Ca共培养的增殖试验。结果表明，与HSC3、HO1myu1、SAS共培养的HNDLEC细胞活力维持时间比不与HSC3、HO1myu1、SAS共培养的要长。某些OSCC可产生淋巴血管生成所需的生长因子。

We have been cleared that the human lymphatic endothelium expresses desmoplakin which is a component of desmosome. In this study, the expression of desmosome-associated proteins ; desmoplakins and plakoglobin in plaque, and desmocollins and desmogleins in core was tested on the oral squamous cell carcinoma (OSCC) in the maxillary antrum and tongue. In the results, 1)lymphatic endothelium in cancer tissues expressed desmoplakins, 2)OSCC in lymphatic vessles overexpressed desmoplakins, and 3)OSCC developed a tendency to lose desmosomal core. In the study for the expression of desmosomal proteins on OSCC cell lines ; HSC2,HSC3,HSC4,HO1myu1,HO1N1,Ca.922,SAS,KB, it was observed that 1)all cell lines did not express desmoglein 1 and 2, 2)KB did not express desmoglein 1-3, 3)all cell lines except for Ca.922 did not express desmocollin 1, 4)HSC4,HO1myu1,HO1N1,SAS rarely express desmocollin 2, 5)all cell lines express desmocollin 3, plakoglobin, and desmoplakin 1 and 2, and 6)in HO1myu1,HO1N1,Ca.922, and SAS, desmoplakin expresses at cell-cell contact but in HSC2,HSC3, and HSC4, desmoplakin diffuses intracellularly. The OSCC may bind to lymphatic vessels with surface exposed desmoplakin overexpressed on cells. Furthermore the OSCC cells in both cell lines and tissues having p53 mutant also intracellulaly overexpressed desmoplakin. The over-expression of desmoplakin may be implicated of the p53 mutation. In the cell growth test for the human neonatal lymphatic endothelial cells (HNDLEC), it was showed that HNDLEC could not proliferate without vascular endothelium growth factor (VEGF) and insulin-like growth factor (IGF). The proliferative test of HNDLEC in co-culture with HSC2,HSC3,HSC4,HO1myul,HO1N1,Ca.922,SAS, and KB, it was suggested that the maintenance of cell viability of HNDLEC is longer in co-culture with HSC3, HO1myu1, SAS than in culture without them. Some OSCC may produce the growth factors which need to the lymphatic angiogenesis.