Laser Micromanipulation Using Microtools at Micro Fluidic Environment

(1)贴壁微工具的利用：我们将酵母细胞贴壁，在没有激光直接照射的情况下成功地将酵母细胞运输到靶细胞上。(2)微工具的制备：利用光密联树脂，通过组装微珠，制备了荧光微工具、彩色微工具、功能微工具等多种微工具。(3)利用微工具操作微生物：将微生物固定在微工具上，通过高速捕获光交联树脂制成的微工具来间接操作微生物。(1)微刀具的制备方法：通过调节光密合树脂的密度和紫外光的照射，在芯片内部制备微刀具。(2)微流体环境的设计与制作：制作微芯片并将细胞固定在芯片内。控制流体环境以测量细胞反应。对酵母细胞进行了培养和荧光反应实验。使用这种技术可以进行单细胞分析。(3)微通道内流体状态的控制：利用热敏水凝胶，通过加热凝胶化的方法，设计并制作了微通道。微通道内的流动状况易于控制。(1)激光微操纵的稳定操作：推导了多个微刀具沿给定轨迹的稳定条件。分析中考虑了布朗运动的影响。成功地实现了多目标的稳定轨迹控制。我们还测量了光阱力。通过实验验证了所提轨迹控制方法的有效性。(2)激光微操纵的位置和方向控制：我们将三个微珠组装成三角形，并独立控制其位置和方向。少

(I)Design and Fabrication of Microtools(1)Utilization of adherent microtools : We adhered the yeast cell and succeeded in transportation of it without direct laser irradiation to the target cell.(2)Fabrication of microtools : Using photoclosslinkable resin, we made several microtools, such as fluorescent microtool, colored microtool, and functional microtools by assembling microbeads.(3)Manipulation of microorganism using microtools : A microorganism was immobilized on the microtool, and was manipulated indirectly by trapping the microtool made of photoclosslinkable resin in high speed.(II)Preparation of Microtools and Environmental Control(1)Method of preparation of microtools : We fabricated microtools inside the chip by adjusting the density of photoclosslinkable resin and irradiation of UV light.(2)Design and fabrication of micro fluidic environment : We made a micro-chip and immobilized cells in it. The fluid environment was controlled for measurement of cell reaction. We performe … More d experiments of culture and fluorescent reaction of yeast cells. A single cell analysis was possible with this technology.(3)Control of fluid condition in the micro-channel : We designed and fabricated a micro-calve using thermo sensitive hydro gel, which is gelled by heating. The flow condition in the micro-channel was controlled easily.(III)Stabilization of Position and Orientation Control of Target Object Using Microtools(1)Stable manipulation by laser micromanipulation : We derived stability condition of the multiple microtools along each given trajectories. Effect of the Brownian motion was considered in the analysis. We succeeded in trajectory control of multiple targets stably. We also measured the optical trap force. Effectiveness of the proposed trajectory control method was confirmed through the experiments.(2)Position and orientation control by laser micromanipulation : We assembled three microbead in triangular shape and controlled its position and orientation independently. Less