The development of an optimal mushroom farming system for the edible mushroom, using the bioelectric potential of the mushrooms as an index, with a LED as a source of illumination

菌类养殖最佳生长条件的科学研究，如培养基、温度、湿度、光照等，一直进展缓慢。通过观察与蘑菇生长密切相关的生物电势，研究了光刺激条件（波长、亮度和光照方式）与蘑菇形态发生特性的关系。从工程角度对最佳耕作条件进行了研究，开发了一种新型光源。通过核磁共振成像对真菌丝线的生长进行了非破坏性的拍摄。此外，在光质量对形态发生的影响方面，通过对生物分子的深入研究，对DNA的表达进行了遗传学研究。本研究的主要内容如下：(1)光刺激因子的系统性变化：改进了用于记录生物电势的精确自动测量系统。(2)极弱自发磁场的测量：利用SQUID测量蘑菇产生的极弱自发磁场，这是一种非破坏性和非侵入性的测量技术。利用测量结果在培养基中研究真菌线的成熟度或菌毛的生长水平的有效性进行了评估。(3)蘑菇养殖现场光源的开发：利用改进的专用简易生长室，在不同光刺激下进行形态发生对比实验。同时还进行了生物电电位传感，以便对蘑菇进行健康检查。研究了利用蘑菇作为生物传感器作为环境监测仪的有效性。目前，对不同光照条件下的形态发生进行了比较研究，包括连续光照、波动光照、脉冲光照等。然而，目前还没有得出明确的结论。(4) MRI横切面照片：每两周非破坏性、非侵入性地拍摄蘑菇从真菌到原基形成的生长过程。研究结果为蘑菇成熟度的研究提供了新的视角。(5)遗传分析：针对光照特征对形态发生的影响，采用DD法检测信使RNA（mRNA）并进行比较分析。少

The scientific study of the optimal growth conditions for mushroom farming, such as culture medium, temperature, humidity, illumination, etc, has been making slow progress. By observing the bioelectric-potential of mushrooms, which is considered to be closely related to the growth, the relationship between light stimulus conditions (wavelength, brightness, and illumination methods) and the morphogenetic properties has been investigated. A new type of light source was developed out of studies of the optimum farming conditions from an engineering standpoint. Photographs were taken non-destructively of the growth of fungal threads by MRI. Furthermore, with regard to the effect of the light quality on the morphogenesis, genetic investigations were made into the expression of DNA by an advanced study of bio-molecules.Here are the outlines of this study :(1)Systematic changes in light stimulus factor :An improvement was made in the accurate auto-measurement system for recording bio-electric … More potentials stimulated by illumination in a purpose-built growth chamber.(2)Measurement of very weak spontaneous magnetic field :The very weak spontaneous magnetic field generated by the mushrooms were measured using a SQUID as a non-destructive and non-invasive measuring technique. The effectiveness of the use of the measured results in the study of ripeness of the fungal threads in the culture medium or growth levels of the pileus was evaluated.(3)Development of a light source at the mushroom farming site :Using the improved purpose-built simple growth chamber, comparative experiments were conducted on morphogenetics with various light stimuli. Bio-electric potential sensing was also undertaken at the same time in order to make health checks on the mushrooms. The effectiveness of the use of mushrooms as a bio-sensor as an environmental monitor was also studied. Currently, a comparison study of morphogenesis under various illumination regimes has been conducted, including continuous illumination, fluctuated illumination, pulsed illumination, etc. However, no clear conclusions have yet been arrived at.(4)Cross sectional photographs taken using MRI :The photographs of the mushroom growth process from fungus through to the formation of primordium have been taken, non-destructively and non-invasively, every two weeks. The results have led to a perspective study of the mushroom ripeness.(5)Genetic analysis :With regard to the influence of the characteristics of illumination on the morphogenesis, the messenger RNA(mRNA) was detected using the DD method and compared for analysis. Less