Genome Approaches to Investigate Metabolic Coordination in Plant Cells

豆科植物根瘤中的碳氮代谢需要共生细菌与植物细胞器之间的相互作用，特别是质体和线粒体中的代谢。 固定氮通过质体和线粒体中嘌呤的从头合成而被同化。 然而，九种途径酶中的每一种都由单个基因编码，这表明每种蛋白质都靶向两种细胞器。 嘌呤代谢将提供一个模型，以评估更普遍的双靶蛋白在植物中的发生。 目的是确定并最终利用介导质体和线粒体之间通信的信号传导机制。

Metabolism of C and N in legume nodules requires interaction between the symbiotic bacteria and plant organelles, particularly metabolism in plastids and mitochondria. Fixed N is assimilated through the de novo synthesis of purines in both plastids and mitochondria. However, each of the nine pathway enzymes is encoded by a single gene, indicating each protein is targeted to both organelles. Purine metabolism will provide a model to assess the more general occurrence of dual-targeted proteins in plants. The aim is to identify and eventually exploit the signalling mechanism(s) that mediate communication between plastids and mitochondria.