Isolation of Denitrifying Phosphorus Removing Bacteria and Application of the Biochemical Information for Optimal Control of Advanced Wastewater Treatment Plant

提出了一种新型的反硝化聚磷微生物筛选装置。在厌氧-缺氧交替条件下进行了筛选工作。仅在厌氧阶段，乙酸作为唯一的有机物。在缺氧阶段，硝酸盐作为最终的电子受体，不添加有机物。由于设备内部是在一种状态到另一种状态的转换阶段用蒸馏水洗涤的，所以在工作过程中醋酸盐和硝酸盐不会同时存在。通过对某城市污水处理厂活性污泥的处理，获得了几种细菌。几乎分离的细菌能够在独特的条件下积累聚磷酸盐和PHB，如厌氧-缺氧交替条件。通过显微镜染色法，有理由定性地假设这些细菌负责加强生物除磷。然而，没有定量观察到这些纯培养物在厌氧条件下与乙酸一起释放磷酸盐，在缺氧条件下消耗磷酸盐。

A brand-new screening equipment was proposed to isolate the denitrifying phosphate-accumulating organisms. A screening work was carried out under the alternating anaerobic-anoxic condition. At only anaerobic phase, acetate was used as a sole organic matter. At the anoxic phase, nitrate as a final electron acceptor and no organic matters were added. Since the inside of equipment was washed with distilled water at the shifting phase from one condition to another condition, acetate and nitrate did not exist simultaneously during the work. As a result of these works using activated sludge in a municipal waste water treatment plant, several kinds of bacteria were obtained. Almost isolated bacteria were able to accumulate polyphosphate and PHB under unique conditions such as alternating anaerobic-anoxic conditions. By microscopy with a staining method it was reasonable to suppose qualitatively that these bacteria were responsible for enhanced biological phosphorus removal. However, it was not quantitatively observed that these pure cultures released phosphate with acetate under anaerobic conditions and consumed phosphate under anoxic conditions.