Study on anti-allergic activity of DNA from immunobiotics and its application for biodefence foods.

最近的兴趣集中在肠道免疫对宿主防御的重要性上，但到目前为止，对其潜在机制还知之甚少。Toll样受体家族通过识别细菌病原体相关分子模式(PAMPs)在防御中发挥重要作用。本研究从食品的生理功能出发，通过TLRs研究了免疫生物乳酸菌(LAB)的免疫调节作用。在猪身上的研究，预计将作为人类模型，已经由免疫生物实验室检查了肠道免疫调节。我们的研究现在证明了肠道中TLRs介导的肠道免疫调节。在此基础上，我们还致力于建立免疫生物实验室的分子免疫分析系统，并从免疫生物中寻找新的免疫刺激DNA序列。体外和体内研究表明，免疫刺激DNA序列具有抗过敏活性。本项目的研究结果可能为进一步阐明免疫刺激DNA激活先天免疫反应的机制提供动力。此外，从实验室中鉴定免疫调节因子，并通过细胞因子网络更好地了解肠道免疫调节，对于食品免疫学的基础研究和预防过敏性疾病的免疫生物食品的开发具有重要意义。

Recent interest has focused on the importance of intestinal immunity for the host defense, but to date, not much has been known about the underlying mechanisms. Toll-like receptor (TLR) family plays an important role in the defense through recognizing bacterial pathogen associated molecular patterns (PAMPs). In this study, the physiological function of food products has investigated the immunoregulatory effects of immunobiotic lactic acid bacteria (LAB) via TLRs. Studies in swine, which is expected as a human model, have been examined intestinal immunoregulation by the immunobiotic LAB. Our research has now demonstrated regulation of intestinal immunity mediated by TLRs in the gut. On the basis of this study, efforts have also been made to develop a molecular immunoassay system for immuobiotic LAB and find novel immunostimulatory DNA sequences from imunobiotics. In vitro and in vivo study demonstrated an anti-allergic activity of immunostimulatory DNA sequences from imunobiotics. The findings in this project may provide impetus to further delineate the activation mechanism of the innate immune response with immunostimulatory DNA. In addition, identification of immunoregulatory factors from LAB, and better understanding of intestinal immune regulation through cytokine networks hold promise in basic research of food immunology and development of "immunobiotic foods" to prevent allergic disease.