Studies on abnormalities of hormone and metabolism in Japanese black cattle with growth retardation

在日本黑牛中经常观察到生长迟缓。犊牛生长迟缓的发生率为3%至5%。我们探讨了生长迟缓的病理生理机制，并试图发展早期诊断的方法。在生长迟缓的牛中，血液甲状腺激素浓度低于正常牛。然而，这是正常甲状腺病态综合征，因此甲状腺功能减退是继发于其他异常。采用气相色谱/质谱联用技术，我们分析了尿代谢谱在牛生长迟缓。气体/质量分析检测到1头乳清酸尿犊牛，但未发现尿苷一磷酸合成酶基因异常;生长迟缓牛具有菌株间的特征谱，但未检测到特定分子的异常。激素方面，生长激素分泌较多，血浆胰岛素样生长因子（IGF-1）浓度较低 ...更多信息 生长发育迟缓牛的血清白蛋白水平高于正常牛。不同品系牛的内分泌异常程度不同，M品系牛GH分泌量较高，IGF-1、T3浓度较低。血浆中T4和皮质醇含量高于H系牛。在生长迟缓的牛中，我们发现许多牛患有凝血因子Xi缺乏症，并且异常基因已经在该地区扩散。另一方面，我们进行了丙酸和精氨酸耐受性试验和代谢谱试验，结果表明，生长迟缓的牛处于容易释放能量的状态，即瘦下来的状态，而不是胖起来的状态。生长迟缓牛血浆IGF-1浓度持续偏低，纵向测定血浆IGF-1浓度可作为生长迟缓的一个指标。此外，我们还可以对牛的罕见疾病、胸腺萎缩、糖尿病、低体温、嗜酸性肌炎、心包间皮瘤和小脑缺陷进行病例报告。少

Growth retardation has been observed frequently in Japanese black cattle. The incidence of growth retardation was 3 to 5 % of calves. We investigate pathophysiology of the growth retardation, and try to develop the method of early diagnosis. In cattle with growth retardation, blood thyroid hormone concentrations were lower than normal cattle. However, this was the euthyroid sick syndrome, so that the hypothyroidism was secondary from other abnormalities. Using gas chromatography/mass spectrometry, we analyzed urinary metabolic profiles in cattle with growth retardation. The gas/mass analysis detected a calf with orotic acid uria, but the abnormality of the gene of responsible enzyme, uridine monophosphate synthase, could not be found. Cattle with growth retardation had a characteristic profile among strains, but the abnormality of particular molecule could not be detected. On hormones, growth hormone secretion was more, and plasma insulin-like growth factor (IGF)-1 concentration was lo … More wer in cattle with growth retardation than normal cattle. Moreover, the degree of endocrine secretion abnormality was different by cattle of strain, the cattle in the M strain had higher GH secretion, lower concentrations of IGF-1, T3. T4 and cortisol in plasma than cattle in the H strain. Of cattle with growth retardation, we found many cattle with blood coagulation factor XI deficiency, and the abnormal gene already became diffused in the area. On the other hand, we carried out propionate and arginine tolerance tests and a metabolic profile test, and results indicated the cattle wit growth retardation were at the condition to release energy easily, namely, the condition to be thin down, not to be fat. Cattle with growth retardation had low plasma IGF-1 concentrations constantly, and the longitudinal determination of plasma IGF-1 concentration can be used as an index of growth retardation. In addition, we could make case reports on rare diseases in cattle, thymic atrophy, diabetes mellitus, low body temperature, eosinophilic myositis, pericardial mesothelioma, and cerebellar deficiency. Less