Gene therapy for the treatment of bone and soft tissue tumors

采用细胞培养和自行建立的实验动物模型，对骨和软组织肿瘤的基因治疗进行了体内外研究。单纯疱疹病毒胸苷激酶基因联合抗病毒药物对骨肉瘤肺转移有明显抑制作用。自杀基因治疗也抑制了乳腺癌骨转移的生长。Decorin是一种富含亮氨酸的蛋白多糖家族，通过基因差异表达方法鉴定为骨肉瘤肺转移相关基因。在动物模型中，转核心蛋白聚糖基因的骨肉瘤细胞的肺转移受到明显抑制。在31例高级别软组织肉瘤中，免疫组织化学染色结果显示核心蛋白的高表达预示着较好的预后，这些结果提示基因治疗对骨和软组织肿瘤及其肺转移的治疗是有效的。

Gene therapy for the treatment of bone and soft tissue tumors was studied in vitro and in vivo using cell culture and an experimental animal model developed originally. Lung metastases of osteosarcoma were inhibited markedly by addition of thymidine kinase gene of herpes simplex virus followed by anti-virus agent. Growth of bone metastasis due to breast cancer was also inhibited by suicide gene therapy. Decorin, small leucin-rich proteoglycan family, was identified as lung metastasis-related gene in osteosarcoma by differential gene expression methods. Lung metastasis of ostesarcoma cells transfected decorin gene were inhibited significantly in an animal model.In 31 high grade soft tissue sarcomas higher expression decorin by immunohistochemical staining showed better prognosis.These results suggest that gene therapy is useful for the treatment of bone and soft tissue tumors and their lung metastasis.