Comparative study on dietary intake of children by food duplicate method in special reference to trace elements in Asian countries

2003~2005年，采用食物重复法对日本、中国和韩国幼儿园儿童膳食中微量元素镉和铅的摄入量进行了比较研究，代表作为众所周知的环境污染物。24-从日本宫城县15所幼儿园的296名4~6岁的男女儿童中收集了100份食物重复样品，中国11所幼儿园的359名儿童和韩国4所幼儿园的113名儿童。除食物复样外，还采集了中国和韩国儿童的现场尿和发样进行微量元素分析。对所有儿童进行身高、体重的人体测量和生活方式、饮食习惯的问卷调查，并对两份食物样品采用国家标准食物成分表进行营养分析。样品经无机酸加热消解后，用原子吸收光谱仪和电感耦合等离子体质谱仪分别测定饲料中钙、镁、磷、钠、钾、氯和铁、锌、铜、锰、镉、铅等微量元素。

Comparative study on the dietary intake of children in Asian countries of Japan, China and Korea were carried at the kindergartens by the food duplicate method from 2003 to 2005 in special reference to trace element of cadmium and lead, representatively as well known environmental pollutants.The 24-hr food duplicate samples were collected from 296 children aged 4 to 6 years of both of boy and girl belonging to 15 kindergartens in Miyagi prefecture in Japan, 359 children of 11 kindergartens in China and 113 children of 4 kindergartens in Korea. In addition to the food duplicate samples, the spot urine and the hair samples were collected from the children in China and Korea in order to make analysis of trace elements. The anthropometric examinations of height and body weight and the questionnaire study on the life style and food habits were worked for all of the children.Nutritional analysis of the duplicate food samples were conducted utilizing the national standard food composition tables for each other. The samples were digested by heating in the presence of mineralacids, and the digests were subjected to analysis for the minerals and trace elements.The measurements of dietary of calcium, magnesium, phosphorus, sodium, potassium, chloride, and the trace elements of iron, zinc, copper, manganese, cadmium, lead and et al. were followed by atomicabsorption and emission spectrometer, and ICP-MS.