Study on the Bio-effect of Ultrasound and Contrast Agents to the Scavenger Cell.

我们研究了超声和造影剂对肝细胞的生物学效应。两个对照USE组中表达CD62P的血小板百分比均增加，且利声显组表达CD62P的血小板百分比更高。我们在两个造影剂使用组中观察到肝细胞空泡化和圆形沉积物。DD-723组在大鼠枯否细胞和少量肝细胞中观察到微泡，而Levovist组在枯否细胞和肝细胞中均未观察到微泡。结论利声显、DD-723、Optizone三种造影剂在脑动脉造影中的应用，在大鼠肝细胞和脑动脉周围的巨噬细胞中产生血小板活化和结构改变，但只有DD-723的微泡被枯否细胞和少数肝细胞摄取。在脑内，超声和造影剂对血脑屏障的一部分巨噬细胞造成损伤。

We studied the bio-effects of Ultrasound and contrast agents to hepatic cells. The percentage of CD62P-expressing platelets increased in both contrast USE groups, and the percentage of CD62P-expressing platelets was greater in Levovist group. We observed vacuolation and round deposits in the hepatocytes in both contrast-USE groups. Microbubbles were observed in the rat Kupffer cells and a few hepatocytes unexpectedly in the DD-723 group, but were found in neither the Kupffer cells nor the hepatocytes in the Levovist group.The bio-effects of the ultrasound and contrast agents to the Macrophage, which is named of Fluorescent Granular Perithelial Cells, around brain arteries can be seen for as increase and damage of Lysosome and edematic changes.Conclusion the contrast agents, Levovist, DD-723 and Optizone, produced platelet activation and structural change in the rat hepatic cells and the macrophages around brain arteries, but only the microbubbles of DD-723 were taken up by the Kupffer cells and a few hepatocytes. In the brain, Ultrasound and contrast agents give the damage of Macrophage which is a part of Blood-Brain Barrier.