Basic and Clinical Studies on Natural Antioxidant Astaxanthin on the Inhibition of Diabetic Complication

本研究将虾青素（一种类胡萝卜素）与其他抗氧化剂如生育酚、生育三烯醇、抗坏血酸或黄酮醇（一种来自松树的多酚混合物）联合应用于糖尿病大鼠，以证实这些组合对氧化应激和糖尿病并发症的有效性。以STZ型糖尿病大鼠为实验对象，证实了以下结果：虾青素与生育酚或生育三烯醇联用对糖尿病大鼠氧化应激有较强的抑制作用。糖尿病大鼠尿液中的8-OHdG生物标志物也低于对照组。然而，在大剂量抗坏血酸与虾青素联合喂养的糖尿病大鼠中，脂质过氧化水平高于对照组。由此可见，大剂量抗坏血酸在体内加速了氧化作用。此外，与虾青素联用可降低生育酚的消耗，同时提高血清和内脏中生育酚的浓度。此外，水溶性黄酮醇与虾青素联合用于抑制糖尿病并发症。结果表明，与黄酮醇联合用药后，大鼠肝脏、肾脏和血清中过氧化脂质含量均低于对照组。白内障的程度也低于对照组。尿液中的8-OHdG生物标志物也低于对照组。由此可见，虾青素与黄烷醇联用对糖尿病大鼠脂质过氧化的抑制作用显著。综上所述，虾青素与生育酚、生育三烯醇或黄酮醇合用可有效抑制糖尿病并发症。糖尿病在发达国家和发展中国家都在增加。对于糖尿病并发症的抑制，希望本研究结果能为糖尿病并发症的预防方法提供参考。人体临床试验将于明年在医院进行。少

In this study, Astaxanthin, a kind of Carotenoid, was used in combination with other antioxidants such as Tocopherol, Tocotrienol, Ascorbic acid or Flavengenol (a mixture of polyphenols from pine) in diabetic rats, to confirm the effectiveness of these combinations against oxidative stress and diabetic complications.The experiments were carried out on STZ diabetic rats, then, the following results were confirmed : In the diabetic rats fed with Astaxanthin in combination with Tocopherol or Tocotrienol the strong inhibition of oxidative stress was observed. The 8-OHdG biomarker in urine of diabetic rats was also lower than that of the control. However, in the diabetic rats fed with Astaxanthin in combination with high dose of Ascorbic acid, the lipid peroxide was higher than that of the control. From this result, it can be concluded that high dose of Ascorbic acid accelerated the oxidation in vivo. Besides, by the combination with Astaxanthin, the comsumption of Tocopherol was reduced, t … More hen the concentration of Tocopherol in serum and internal organs were increased.Moreover, water soluble Flavengenol was used in the combination with Astaxanthin for the inhibition of diabetic complications. Then, the results showed that with the combination with Flavengenol, the lipid peroxide in liver, kidney and serum were lower than that of the control. The degree of cataract was also lower than that of the control. The 8-OHdG biomarker in urine was also lower than that of the control. From these results, Astaxanthin by the combination with Flavangenol, the inhibition of lipid peroxide in diabetic rats was significant.From these above results, Astaxanthin in the combination with Tocopherol, Tocotrienol or Flavengenol was effective for the inhibition of diabetic complications. Diabetes is increasing in developed and developing countries. For the inhibition of diabetic complications, it is hoped that the results in this study will be reference for the prevention method of diabetic complications. The clinical trials in human will be carried out in the coming year, in the hospital.. Less