The association of human papillomavirus with carcinogenesis of oral squamous cell carcinoma.

本研究旨在阐明HPV与口腔鳞状细胞癌的关系，并获得以下结果。(1)我们比较了Takara PCR人乳头瘤病毒检测试剂盒中扩增HPV L1区的PGMY09/PGMY11 L1引物和E6引物。两种引物的敏感性相似，但后者的特异性更好。(2)用Takara引物扩增新鲜冷冻组织、福尔马林固定石蜡包埋切片或拭子样本的dna。在新鲜冷冻组织和拭子样品中观察到相似的灵敏度，但在石蜡包埋切片中灵敏度较低。(3)对肿瘤、肿瘤边缘和正常部位拭子样品洗脱的dna进行分析。肿瘤部位的HPV DNA拷贝数高于其他部位。(4) HPV-18 DNA拷贝数在多发复发患者中较高，在其他患者和正常对照中较低。(5)在转移淋巴结的福尔马林固定石蜡包埋切片的DNA中未观察到HPV-16和HPV-18 DNA。(6) HPV E7 DNA感染患者预后较对照组好。(7)细胞周期调节因子Cdc25A的表达与HPV E7的表达呈正相关，而另一细胞周期调节因子14-3-3的表达与HPV E6的表达呈负相关。

This study was performed to clarify the association of HPV with oral squamous cell carcinoma and the following results were obtained.(1) We compared the PGMY09/PGMY11 L1 primers amplifying HPV L1 region and the E6 primers in Takara PCR Human Papillomavirus Detection Kit. Both primers showed similar sensitivity, but the latter showed better specificity. (2) DNAs obtained from fresh frozen tissues, formalin-fixed paraffin embedded sections, or swab samples were amplified by using the Takara primers. Similar sensitivity was observed in both fresh frozen tissues and swab samples, but sensitivity was low in paraffin embedded sections. (3) We analyzed DNAs eluted from swab samples obtained from tumor, tumor margin and normal part. More HPV DNA copies were observed in tumor part compared with the other parts. (4) HPV-18 DNA copy number was high in the patient with multiple recurrences, but not in the other patients and normal controls. (5) Neither HPV-16 nor HPV-18 DNA was observed in DNAs obtained from formalin-fixed paraffin embedded sections of metastasized lymph nodes. (6) Patients with HPV E7 DNA showed better prognosis compared with the counterparts. (7) Expression of Cdc25A, cell cycle regulator, was positively related to expression of HPV E7 and another cell cycle regulator, 14-3-3, was negatively related to expression of HPV E6.