Molecular and cell biological approach to promote the eruption of impacted teeth

在这个研究项目中，研究了与牙齿表达相关的基因表达，以开发一种新的非侵入性治疗牙齿阻生和延迟性牙萌出的方法。本年度的研究结果如下：1)利用DNA微阵列技术筛选出牙萌出过程中显著上调和下调的基因。2)从编码纤维蛋白1基因突变的马凡综合征患者中分离牙周韧带细胞，并利用逆转录病毒将端粒酶逆转录酶和Bmi-1基因导入这些细胞中。3)发现ADMTSL-1与纤维蛋白相关。4)建立了可再生诱导牙根吸收的体内系统。在该系统中，在紫外线照射和/或风干后，拔除下颌磨牙并重新植入SCID小鼠体内。5)研制了DNA芯片Perio gen芯片。这是基于人牙周膜细胞的基因表达谱。6)检测了釉原蛋白作为Emdogain的生物活性底物对牙根吸收的抑制作用。釉原蛋白对破牙本质牙根吸收有抑制作用。7)这种抑制作用是通过抑制RANKL的表达实现的。

Gene expression regarding the tooth expression was examined in this research project to develop a novel non-invasive treatment for tooth impaction and delayed tooth eruption. The following are research results of this year.1) Genes which were significantly up-regulated and down-regulated by the tooth eruption were clarified by using DNA microarray2) Periodontal ligament cells were isolated from the patient with Marfan syndrome that has mutation in the gene encoding fibrillin 1. Teromerase reverse transcriptase and Bmi-1 were introduced into these cells by using retrovirus.3) ADMTSL-1 was discovered to associate with fibrillin 1.4) In vivo system, in which root resorption was induced reproducibly, was established. In this system, the mandibular molars were extracted and reimplanted to SCID mice after UV irradiation and/or air dry.5) DNA chip, Perio gen chip, was developed. This is based on the gene expression profile of human periodontal ligament cells.6) The inhibitory effect of Amelogenin known as bioactive substrate of EMDOGAIN for tooth root resorption was examined. Amelogenin was found to have inhibitory effect on odontoclastic root resorption.7) This inhibitory effect was via suppressing RANKL expression.