Periodontal regeneration by BMP application to root surface and scaffold of periodontal ligament

第一个实验的目的是评价rhBMP-2在牙本质表面应用后不同牙本质调理剂的效果。牙本质块用EDTA、四环素- hci或柠檬酸溶液脱矿3分钟，用rhBMP-2溶液浸泡。将牙周韧带细胞接种于各牙本质块上，测定附着细胞的ALP活性。结果表明，EDTA脱矿组具有较高的ALP活性。实验二将各牙本质块移植到大鼠腭结缔组织中。组织学检查显示，EDTA脱矿组和rhBMP-2应用组的硬组织形成明显多于其他各组。第三个实验采用EDTA和rhBMP-2对牙本质块进行脱矿处理，植入大鼠腭结缔组织。给予大鼠双膦酸盐抑制破骨细胞。结果表明：硬膜的形成和牙本质吸收均明显减少。提示成骨细胞和破骨细胞之间的偶联系统可能在牙本质表面硬组织形成中起重要作用。第四项研究的目的是评价EDTA脱矿、BMP-2溶液和胶原水凝胶应用于牙根本质表面后牙周开裂模型的再生情况。新骨形成明显多于其他任何组。然后对1壁骨下缺损的牙周再生进行评价。新骨和新牙骨质形成明显多于对照组。冠状区多见从原有牙骨质中分离出来的新牙骨质，可能由牙龈结缔组织形成。这些结果表明了再生过程的新方面。结论：BMP-2溶液和胶原水凝胶应用于牙根本质表面有利于牙周组织再生。这种方法可能是治疗大面积牙周缺损的有效再生方法。少

The aim of the first experiment was to evaluate the effect of different dentin conditionings followed rhBMP-2 application on dentin surface. Dentin blocks were demineralized with EDTA, tetracycline-HCI or citric acid solutions for 3 minutes, and were soaked with rhBMP-2 solution. Periodontal ligament cells were seeded on each dentin blocks, and ALP activity of attachment cells were assessed. The results showed that the groups of EDTA demineralization had higher ALP activity. In the second experiment, each dentin blocks were transplanted into palatal connective tissue of rats. The histological examinations showed that the groups of EDTA demineralization and rhBMP-2 application had significantly more hard tissue formation than any other groups. In the third experiment, dentin blocks were demineralized with EDTA and rhBMP-2 applied, and then implanted into palatal connective tissue of rats. Bisphosponate was administered to rats for osteoclast suppression. The results showed that hard tis … More sue formation and dentin resorption were decreased. It suggested that coupling system between osteoblast and osteoclast may play an important role in hard tissue formation on dentin surface. The aim of fourth study was to evaluate periodontal regeneration in dehiscence model after EDTA demineralization, application of BMP-2 solution and collagen hydrogel to root dentin surfaces. New bone formation was significantly greater than any other group. Then, periodontal regeneration in 1-wall infrabony defects was evaluated. New bone and new cementum formation was significantly greater than in the control group. New cementum isolated from original cementum was frequently observed in the coronal area, and it might be formed by gingival connective tissue. These results suggest that new aspects of the regenerative process are introduced.It was concluded that the application of BMP-2 solution and collagen hydrogel to root dentin surface was conducive to periodontal tissue regeneration. This procedure may be effective regenerative therapy for large periodontal defects. Less