Induction of reparative dentin by Enamel Proteins

我们已经证明，釉质基质衍生物(EMD)促进修复性牙本质的形成与牙髓损伤后牙本质特异性因子的顺序表达有关。值得注意的是，在这些研究中，在应用EMD后不久，在创面区域的牙髓细胞中检测到了猪成釉蛋白的表达。为了确定AMBN在牙本质形成中的作用，并探索AMBN在牙髓愈合中的潜在应用，我们通过组织形态计量学和免疫组织化学方法，研究了将重组融合AMBN应用于暴露的牙髓组织是否能够促进牙髓愈合和继发性牙本质形成。在rAMBN处理的牙齿中，在应用部位观察到大量新形成的修复性牙本质，完全桥接牙髓伤口。在Ca(OH)2处理的牙齿中也观察到牙本质的形成，但修复性牙本质的数量明显少于rAMBN处理后。免疫组织化学证实，新形成的硬组织类似牙本质。结果表明，rAMBN作为一种生物活性的牙髓敷料剂，在促进牙髓创伤愈合和牙髓切断术后的修复性牙本质形成方面具有潜在的潜力。

We have shown that enamel matrix derivative (EMD) enhanced reparative dentin formation was associated with a sequential expression of dentin-specific factors in the wounded pulp. Notably, in these studies, expression of pig ameloblastin was detected in pulpal cells in the wound area shortly following EMD application. To confirm a functional role for Ambn in dentinogenesis and to explore the potential use of Ambn for pulpal healing, we investigated if application of recombinant fusion Ambn (rAmbn) onto exposed pulp tissue could enhance pulpal healing and secondary dentin formation by histomorphometry and immunohistochemistry. In rAmbn-treated teeth, a substantial amount of newly formed reparative dentin was observed at the application site, completely bridging the pulpal wound. Dentin formation was also observed in Ca(OH)2 trated teeth; however, the amount of reparative dentin was significantly smaller than after rAmbn treatment. Immunohistochemistry confirmed that the new hard tissue formed was similar to dentin. The results suggest potential for rAmbn as a biologically active pulp-dressing agent for enhanced pulpal wound healing and reparative dentin formation after pulpotomy procedures.