Applications of molecular nanotechnology for human disease using DNA-encode based gene expression analysis

尽管DNA微阵列可以同时监测数千个基因的表达，但很难比较不同生物（即人类和病原体）中不同平台的微阵列结果。我们建立了一个人类传染病基因表达分析应用程序，可以在一个平台上分析宿主和病原体的基因表达。在我们的系统中，目标特异性序列被协调到DNA编码号中，即DNA编码分析（DEAN），允许在单一平台上进行可重复的杂交，无论生物体如何。我们将该方法应用于病毒感染模型，并证明了DEAN系统原则上是一种基于dna的计算机的可能性，该计算机可用于理解复杂的人体免疫调节系统对病原体的反应。本方法不适用于基因表达定量分析。相反，它将为生命系统和人类疾病的生物计算研究提供积极影响的见解。

Despite the fact that DNA microarrays can simultaneously monitor the expression of thousands of genes, it is difficult to compare microarray results of different platforms in various organisms (i.e. humans and pathogens). We established an application for gene expression analysis of human infectious disease which enables to analyze gene expression in both host and pathogen on a single platform. In our system, the target-specific sequences were concerted into the DNA code number, i.e., DNA encoding-based analysis (DEAN) which permits reproducible hybridization, regardless of organisms, on a single platform. The wet experiments were configured as robotic benchtop workstation We applied the method in a viral infection model, and demonstrated the possibility that the DEAN system is in principle a DNA-based computer which has applications for understanding the complex human immunoregulatory system in response to pathogens. This method is not application of quantitative gene expression analysis. Instead, it will provide insights into positive impact on research of biocomputing in living system and human diseases.