Development of trace analysis of perfluorochemicals in human biological samples and assessment of exposure

2005 ~ 2007财政年度对生物样品中全氟化学品的痕量分析和人体暴露评估进行了调查。2005财政年度：对人体血浆中全氟化合物的测定，研究了柱切换液相色谱-串联质谱（LC-MS/MS）的分析方法，并将该方法应用于母体血液和脐带血中全氟化合物的分析。该方法对人体血浆中PFCs的监测具有高度的灵敏度和选择性。获得了母体血和脐带血PFCs浓度的比例关系。推测pfc从母体血液转运到胎儿血液。2006财政年度：为了阐明人体血液中高频检测到的全氟化合物的暴露来源，研究了采用超临界流体萃取（SFE）和LC-MS/MS相结合的分析方法测定房屋粉尘样品中的全氟化合物。采用SFE预处理的分析方法成功地应用于室内粉尘的分析，具有较高的灵敏度和重复性。与其他环境样本相比，室内粉尘中全氟化合物的浓度明显较高，被认为是人类主要的暴露源之一。2007财年：基于母亲脐带血中PFCs向胎儿的转运，采用固相萃取和LC-MS/MS对母乳中PFCs进行了高灵敏度的分析。结果表明，母乳是pfc从母体向婴儿传递的途径之一。

For the development of the trace analysis of perfluorochemicals(PFCs) in biological samples and evaluation of human exposure were investigated by the 2005〜2007 fiscal year.The 2005 fiscal year : For PFCs measurement of the human plasma, the analytical method using the column switching liquid chromatography-tandem mass spectrometry(LC-MS/MS) has been studied, and the method was applied to the analysis of PFCs in maternal blood and umbilical cord blood. The method is highly sensitive and selective for the monitoring of PFCs in human plasma. The proportional relation of concentration of PFCs between maternal blood and umbilical cord blood was obtained. The transport of PFCs from maternal blood into fetal blood was speculated.The 2006 fiscal year: In order to elucidate the exposure source of PFCs, which have been detected in human blood with high frequency, the analytical method by use of supercritical fluid extraction (SFE) and LC-MS/MS was investigated for measurement of PFCs in house dust samples. The developed analytical method which used SFE for the pretreatment was successfully applied to analysis of house dusts with high sensitivity and repeatability. In comparison with other environmental samples, the concentration of PFCs in house dust is remarkably higher and considered as one of the major human exposure source.The 2007 fiscal year : Based on the transport of PFCs to the fetus through the umbilical cord blood from the mother the analytical method of PFCs in mother's milk with highly sensitivity was carried out with solid phase extraction and LC-MS/MS. In results, it was indicated that the mother's milk was one of the transport pathway of PFCs to the infant from the mother.