Developmental study for hybrid artificial lymph vessels:its potential role of permeability

淋巴系统的主要功能之一是将血浆蛋白从组织空间返回到血流。此外，淋巴系统具有独特的机制，在淋巴液的运输过程中，淋巴管和淋巴结可以调节淋巴液的含量。这些机制被认为是重要的了解淋巴生理以及与淋巴疾病相关的病理生理。在本项目中，为了开发混合型人工淋巴管，我们研究了淋巴管的内皮细胞和平滑肌细胞的功能，重点是渗透性。在离体大鼠淋巴管管腔内，NaF_1和FITC-葡聚糖4400（4kD）可穿透淋巴管壁。FITC-葡聚糖12000（12 kD）在离体大鼠淋巴管管腔内有轻度穿透淋巴管壁的现象，而FITC-葡聚糖71200（70 kD）不穿透淋巴管壁。在将CHAPS或TNF α注入淋巴管后，FITC-葡聚糖4400和12000的净流量增加，但71200没有增加。TNFα可引起培养的淋巴管内皮细胞F-actin重排。这些结果表明，小分子亲水性物质（4400）是可渗透的从管腔内到外的间隙的孤立的淋巴管和内皮细胞表面结构可能发挥屏障作用的有效渗透性的大分子亲水性物质（4400至12000）通过淋巴管壁。来自细胞内糖原和/或通过淋巴管平滑肌中的GLUT/SGLT的葡萄糖参与了离体大鼠胸导管泵活性的调节，细胞内线粒体K_2<ATP>可能部分地作为与线粒体Ca^2+摄取相关的机械功能的调节剂。在这个项目中，我们展示了关于“混合人工淋巴管的开发研究：其渗透性的潜在作用”的重要问题。

One of the major functions of the lymphatic system is to return plasma proteins from the tissue space to the blood stream. In addition, the lymphatic system has a unique mechanism that lymphatics and lymph nodes could modulate lymph contents during transport of lymph. These mechanisms are considered to be important for understanding lymphatic physiology as well as the pathophysiology associated with lymphatic disorders. In this project, for development of hybrid artificial lymph vessels, we have studied the functions of endothelial cells and smooth muscle cells of the lymph vessels focusing on the permeability. NaF1 and FITC-dextran 4400 (4kD) in the intraluminal space of isolated rat lymph vessels penetrated the wall of lymph vessels. FITC-dextran 12000 (12kD) in the intraluminal space of isolated rat lymph vessels slightly passed through the lymphatic wall, while FITC-dextran 71200 (70kD) did not penetrate the wall. After administration of CHAPS or TNF≪ into the lymph vessels, the net flux of FITC-dextran 4400 and 12000, but not 71200, augmented. TNFα caused a re-arrangement of F-actin in cultured lymphatic endothelial cells. These results suggest that small molecular hydrophilic substances (〓 4400) are permeable from the intraluminal to extrahiminal space of isolated lymph vessels and that the endothelial cell surface structure may play a barrier role in the effective permeability of large molecular hydrophilic substances (4400 to 12000) through the wall of the lymph vessels. Glucose derived from intracellular glycogen and/or through GLUT/SGLT in lymphatic smooth muscles contributes to the regulation of pump activity of isolated rat thoracic ducts, and that mitochondrial K_<ATP> in the cells may partially serve as a modulator of the mechanical functions associated with mitochondrial Ca^<2+> uptake. In this project we demonstrated important issues regarding "Developmental study for hybrid artificial lymph vessels: its potential role of permeability".