Effect of polyphenol-containing azuki bean extract on blood pressure and stroke in an experimental model of hypertension

高血压被认为是心肌梗死、中风和肾脏损害的主要危险因素，而且还被证明具有促炎作用，增加了超氧化物(O_2-)等活性氧物种的形成。此外，巨噬细胞等免疫活性细胞的渗透被认为在高血压的发病机制中发挥了作用。虽然赤豆(Vigna Angularis)被认为是最重要的农作物之一，但人们对赤豆饮食对高血压进展的有益影响知之甚少。在本研究中，我们研究了含有清除自由基活性的多酚的赤豆提取物(ABE)对高血压模型大鼠的降压作用，以及ABE是否影响慢性高血压大鼠主动脉和肾脏中O2的产生和巨噬细胞的浸润。实验一：易卒中自发性高血压大鼠饲喂0%和0.9%ABE-Containin…更多的G节食。测定尾收缩压(SBP)和主动脉、肾组织氧分压(O2)。ABE治疗的SHRSP与未治疗的SHRSP相比，SBP的升高没有受到抑制。另一方面，经ABE处理的SHR经NADPH刺激后的O2水平较未处理的SHR显著降低。实验Z自发性高血压大鼠(SHR)和正常血压的Wistar京都大鼠(WKY)分别饲喂0%和0.8%的ABE饲料。测定心脏和肾脏的尾部收缩压和巨噬细胞动力学。经ABE治疗8周后，经ABE治疗的SHR的SBP升高明显低于未治疗的SHR。免疫组织化学分析显示，经ABE治疗的SHR大鼠心脏和肾脏的巨噬细胞数较未治疗的SHR明显减少，提示ABE可减轻SHR模型大鼠SBP、O2的升高和巨噬细胞的浸润。较少

Hypertension is known to be a major risk factor for myocardial infarction, stroke, and renal damage, and has also been shown to have proinflammatory actions, which increase the formation of reactive oxygen species such as superoxide (O_2-). In addition, infiltration by immunocompetent cells such as macrophages is thought to play a role in the pathogenesis of hypertension. Although the azuki bean (Vigna angularis) is known to be one of the most important crops, little is known about the beneficial effects of a diet of azuki beans on the progression of hypertension.In the present study, we investigated the effect of treatments of azuki beans extract (ABE) containing polyphenols with radical scavenging activities on high blood pressure in a rat model with hypertension, and whether the ABE affects O_2 production and macrophages infiltration in the aorta and kidney in chronic hypertension. Experiment 1 : Stroke-prone spontaneously hypertensive rats (SHRSP) were fed 0% and 0.9% ABE-containin … More g diets. Tail systolic blood pressure (SBP) and O_2 production in the aorta and kidney were examined. The elevation of SBP in the ABE-treated SHRSP was not suppressed compared with the untreated SHRSP. On the other hand, The NADPH-stimulated O_2 levels in the ABE-treated SHR was decreased significantly, compared with the untreated SHR. Experiment Z Spontaneously hypertensive rats (SHR) and control normotensive Wistar Kyoto rats (WKY) were fed 0% and 0.8% ABE-containing diets, respectively. Tail SBP and macrophage kinetics in the heart and kidney were examined. After 8 weeks of ABE treatment, the elevation of SBP in the ABE-treated SHR was significantly suppressed compared with the untreated SHR. In the immunohistochemical analyses, the number of macrophages in the heart and kidney in the ABE-treated SHR showed a significant decrease in number compared with the untreated SHR.In conclusion, these results suggested that ABE attenuated the elevation of SBP, O_2 production and macrophage infiltration of this SHR model. Less