腎糸球体の再生過程における糸球体内皮細胞の役割に関する検討

为了揭示人类肾小球发生的过程，我们研究了胎儿和婴儿肾脏中肾小球的发育过程和CD31、CD34、FB21、α-平滑肌肌动蛋白(alfa-SMA)和neparin的染色。我们观察了四个发育期[V期、S期、C期和M期]肾小球的发育过程以及各组肾小球CD31、CD34、Fb21、α-SMA和肾小球蛋白的表达。A组V期比例最高，20周后V期比例逐渐下降。B、C组S、C期细胞比例高于D、E、F组，F组M期细胞比例最高，孕25周以上胎儿肾脏内皮细胞CD31、CD34、FB21染色相似。α-SMA染色的肾小球系膜细胞首先出现在S形体的下裂。与之类似，肾小管上皮细胞上皮细胞也同样染色，在S形小体中最早见弱阳性染色。这些结果提示肾小球成熟为…。从妊娠25-34周的V期进入M期，胎儿内皮细胞的成熟在25周开始，35周完成。另一方面，为了明确血清肝细胞生长因子浓度和肾组织肝细胞生长因子受体表达是否可以预测肾功能不全的预后，我们检测了无新月体形成和有新月体形成的过敏性肾炎患者血清肝细胞生长因子浓度和肾小球系膜细胞生长因子受体、α-平滑肌肌动蛋白的表达。根据第一次活检的病理结果，这些患者被分为两组。第1组ISKDC I或II级20例，第2组ISKDC III级、VI级或V级35例。肾小球和间质α-SMA染色平均积分2组明显高于1组，第2次活检时肾小球和间质α-SMA染色平均积分低于第1组。提示首次活检肾小球和间质α-SMA表达可预测HSPN患儿的预后，肾小球c-Met表达可能与肾脏再生有关。较少

To reveal the human glomerulogenesis, we evaluated the process of development of glomeruli and staining for CD31, CD34, FB21, alfa-Smooth Muscle Actin (alfa-SMA), and nephrin in fetal and infant kidneys. We investigated the process of glomerular development in four developmental stages[V-stage, S-stage, C-stage and M-stage]and glomerular CD31, CD34, FB21, alfa-SMA, and Nephrin staining in each group. V-stage proportion was highest in Group A. After 20 weeks, V stage proportion gradually decreased. S- and C-stage proportions were higher in Groups B and C than in Groups D, E and F. M-stage proportion was highest in Group F. CD31, CD34 and FB21 were stained similarly in for endothelial cells in the kidneys of fetuses at over 25 weeks of gestation. Alfa-SMA stained in mesangial cells were found to the lower clefts of the S-shaped bodies first of all. Similarly Nephrin stained in epithelial cells were first observed in S-shaped body weakly. These results suggest that the glomerulus matures … More to M-stage from V stage from 25-34 weeks of gestation and that maturation of endothelial cells starts at 25 weeks and is completed by 35 weeks in the fetus. Epithelial cells and mesangial cells are appeared first at the S-stage.On the other hand, to clarify whether serum concentration of HGF and renal HGF receptor (HGFR) expression can predict the prognosis of renal dysfunction, we evaluated serum concentration of HGF and the expression of HGFR-MET and a -SMA in HSPN patients without crescent formation and with crescent formation. These patients were divided into two groups based on pathological findings on the first biopsy. Group 1 consisted of 20 patients with ISKDC I or II, while group 2 consisted of 35 patients with ISKDC III or VI or V. The mean scores of glomerular and interstitial alpha-SMA staining in group 2 were significantly higher than those in group 1. At second biopsy, the mean scores of c-Met positive staining in group 2 were lower than those in group 1. Our results suggest that the expression of glomerular and interstitial alpha-SMA at first biopsy can predict the prognosis of children with HSPN and the expression of glomerular c-Met at second biopsy may be associated with the reproduction of kidney. Less