Effect of rinsing by sugar solution on oral malodor of tube fad patients

1. 健康的年轻人被要求用10%的葡萄糖溶液漱口。测定漱口前后口腔空气中VSC含量。因此，用葡萄糖溶液冲洗时，VSC浓度明显低于对照组。同一受试者的唾液样本在37℃厌氧条件下用2%葡萄糖或山梨醇孵育90min。孵育后测定硫化氢（H_2S）产率。因此，唾液中含有葡萄糖的H2S含量明显低于对照组。此外，我们还利用实时荧光定量PCR法测定了唾液样品中总细菌、T、连翘和梭杆菌的细胞数。在添加葡萄糖或山梨醇和对照的所有条件下，连翘对总细菌的比例不变。从这些结果来看，糖的供应应该是有效的减少口腔空气中的VSC。进一步说明，这种现象的发生不仅与糖发酵对低pH条件下厌氧微生物的灭活有关。我们从血管链球菌中纯化了产H2S酶。在加糖和不加糖的情况下测定其活性。因此，葡萄糖对活性没有影响。有人认为葡萄糖对酶没有影响。我们测定了某护理机构有管喂养和无管喂养老年人口腔空气中的VSC。两组的口腔状况相匹配。因此，VSC浓度无显著性差异。然而，管饲老年人VSC水平与口腔疾病之间存在高度相关性。结果表明，在整体口腔卫生控制成功的情况下，管饲不是引起口腔异味的原因，但在某些口腔卫生条件较差的情况下，VSC水平升高，特别是管饲组。

1. Healthy young adults were ordered to rinse their mouth with 10% glucose solution. Before and after rinsing, VSC in mouth air were determined. Consequently, in rinsing with glucose solution, VSC concentrations was significantly lower than that in the control.Saliva samples from same subjects were incubated for 90min at 37℃ under anaerobic condition with 2% glucose or sorbitol. After the incubation, hydrogen sulfide (H_2S) production was measured. Consequently, the amounts of H2S from saliva with glucose were significantly lower than the control. Furthermore, we determined the cell number of total bacteria, T, forsythia and Fusobacterium in the saliva samples using real-time PCR method. The proportion of T forsythia against total bacteria was constant in all conditions (glucose or sorbitol added and control). From those results, sugar supply was supposed to be effective for reduction of VSC in mouth air. Further, it was suggested that the phenomenon occurred by not only inactivation of anaerobic microbe under low pH condition by sugar fermentation.2. We purified H2S producting enzyme from Streptococcus anginosus. The activities were determined with and without glucose. Consequently, there was no effect of glucose on the activities. It was suggested that the glucose did not effect on the enzyme.3. We determined VSC in mouth air of elderly with and without tube feeding in a nursing institution. The two groups were matched by the oral conditions. Consequently, there was no significant difference in VSC concentration. However, the high correlations between VSC levels and oral malconditions in the elderly with tube feeding. From the results, it was supposed that tube feeding was not causative for oral malodor in case the whole oral health control was successful, but if the some oral conditions were poor, VSC levels got higher especially in the subjects with tube feeding.