Nerve protective action by kynurenic acid treatment in a rat model of photochemically induced thrombosis causing focal cerebral ischemia

我们以前证明了激活区的存在，在同侧感觉运动皮层周围地区的脑梗死，使用大鼠模型的局灶性光化学诱导血栓形成（PIT）。使用这个模型，我们接下来使用二维聚丙烯酰胺凝胶电泳（2D-PAGE）和肽质量指纹（PMF）分析的图像来筛选激活区域中上调的关键蛋白。2D-PAGE和PMF鉴定出层粘连蛋白受体1、载脂蛋白E、烯醇化酶2、Stathmin 1和胶质细胞酸性蛋白δ（GFAP）为主要蛋白质。GFAP表达的皮质区域周围的病变增加，从48至96小时后PTI作为证实的免疫detector.In结论，我们表明，层粘连蛋白，载脂蛋白E，烯醇化酶，Stathmin和GFAP是重要的蛋白质，从偏瘫大鼠血栓模型的恢复。特别是，结果表明，与这些蛋白质相关的星形胶质细胞在PIT模型中的行为恢复中起着重要作用。

We demonstrated previously the presence of activated areas in the ipsilateral sensorimotor cortex surrounding areas of cerebral infarction, using a rat model of focal photochemically-induced thrombosis (PIT). Using this model, we next used the images of two-dimensional poly-acrylamide gel electrophoresis (2D-PAGE) and peptide mass fingerprinting (PMF) analysis to screen key proteins upregulated in the activated area. 2D-PAGE and PMF identified Laminin receptor 1, Apolipoprotein-E, Enolase 2, Stathmin 1 and Glial fibrillary acidic protein delta (GFAP) as the main proteins. GFAP expression in the cortical area surrounding the lesion increased from 48 to 96 h after PTI as confirmed by immunodetection.In conclusion, we showed that Laminin, Apo-E, Enolase, Stathmin and GFAP are important proteins for recovery from hemiplegia in the rat thrombosis model. In particular, the results showed that astrocytes, which are related to these proteins, play an important role in behavioral recovery in the PIT model.