Dose the stem cell transplantation promote motor recovery after a brain lesion induced by photochemically initiated thrombosis?

干细胞移植是否能促进光化学引发的血栓形成引起的脑损伤后的运动恢复？

• 批准号: 21650143
• 负责人: ABO Masahiro
• 金额: $ 2.1万
• 依托单位: Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21650143/
• 关键词: 脳卒中; ES細胞; アストロサイト; 機能回復; 神経幹細胞移植; 脂肪組織由来間葉系幹細; 回復; モデルラット; 脂肪組織由来間葉系幹細胞

We selected the rat model of a brain lesion induced by photochemically initiated thrombosis(PIT) through platelet aggregation. The technique involved in this model is simple enough to design the brain lesion as focal and uniform, and the hemiparesis of the hindlimb, induced by injury to the unilateral motor cortex, recovers completely by the 10th day with few individual differences after operation. The purpose of this study was to examine whether using the stem cell transplantation or the mesenchymal stem cell transplantation promotes motor recovery after brain damage in this PIT model. After recovery of left hindlimb function caused by a right sensorimotor cortex lesion, a lesion was induced photochemically in the left sensorimotor cortex as a second PIT and the beam-walking score was recorded for right hindlimb function. As a result, these two methods were not able to promote motor recovery after brain damage in this PIT model.

我们选择了光化学引发血栓形成（PIT）通过血小板聚集引起的脑损伤的大鼠模型。该模型所涉及的技术足够简单，可以将脑损伤设计为局灶性和均匀的，并且由单侧运动皮层损伤引起的后肢轻偏瘫在术后第10天完全恢复，个体差异很小。本研究的目的是检查在该PIT模型中使用干细胞移植或间充质干细胞移植是否促进脑损伤后的运动恢复。右感觉运动皮层损伤引起的左后肢功能恢复后，在左感觉运动皮层中光化学诱导损伤作为第二PIT，并记录右后肢功能的梁行走评分。因此，这两种方法在该PIT模型中不能促进脑损伤后的运动恢复。

项目成果

Proteomic Assessment of Important Proteins for Motor Recovery in a Rat Model of Photochemically-Induced Thrombosis

光化学诱导血栓形成大鼠模型中运动恢复重要蛋白质的蛋白质组学评估

• 发表时间: 2009
• 期刊: The Journal of Applied Research
• 作者: Hideki Yamauchi;Kouhei Miyamura;Masahiro Abo
• 通讯作者: Masahiro Abo

脳損傷モデルとその解析

脑损伤模型及其分析

• 发表时间: 2010
• 作者: 安保雅博;山内秀樹
• 通讯作者: 山内秀樹