Integrated and Fictionalized Nanoparticles in 3-dimentional nanospase

采用化学气相沉积(CVD)方法，以铁蛋白分子衍生的铁纳米颗粒为原料，在99.98%的铂片(20×20×1 mm)上制备了碳纳米管。用拉曼光谱和透射电子显微镜对所合成的碳纳米管进行了表征。在合成碳纳米管后，在铂电极表面观察到两个拉曼位移峰，分别对应于G-(约1580 cm~(-1))和D-(约1350 cm^~(-1)&gt；)，表明碳纳米管存在于电极表面。单个碳纳米管的直径约为5~10 nm，可归类为多壁碳纳米管(MWCNTs)。将在铂电极(MWCNTs/Pt)上合成的MWCNTs立即浸泡在葡萄糖氧化酶(GOx)溶液中，将这些酶固定在MWCNTs/Pt电极表面。将GOx固定在多壁碳纳米管/铂电极上后，GOx的催化氧化电流从-0.45V(vs.Ag/AgCl/sat‘d KCl)增大，接近黄素腺嘌呤二核苷酸(FAD)作为GOx修饰基在生理pH值下的氧化还原电位。果糖脱氢酶固定的多壁碳纳米管/铂电极在-0.15V(vs.Ag/AgCl/sat‘d KCl)处有一清晰的催化氧化电流，接近血红素c作为脱氢酶修饰基的氧化还原电位。从催化电流与底物的关系图分析，果糖浓度的校准范围高达约40mmoldm~(-3)，表观米氏常数为11(±)μdm~(-3)~(-3)；关于酶与多壁碳纳米管之间的相互作用和直接多相电子转移反应的进一步研究正在进行中，以开发传感器应用和葡萄糖-氧气生物燃料电池。

Carbon nanotubes(CNTs)was synthesized on a platinum plate(99.98 %, 20×20×1 mm)by the chemical vapor deposition(CVD)method using iron nanoparticles derived from ferritin molecules. The synthesized CNTs were characterized by Raman spectroscopy and TEM. Two Raman shift peaks corresponding to G-(ca. 1580 cm^<-1>)and D-bands(ca. 1350 cm^<-1>)were observed on the platinum electrode surface after the synthesis of the CNTs, which indicated that CNTs were present on the electrode surface. The diameters of individual CNTs were evaluated to be ca. 5 to 10 nm, which could be classified as multi-walled CNTs(MWCNTs).The MWCNTs synthesized on the Pt plate (MWCNTs/Pt)electrode were immediately immersed into solutions of glucose oxidase (GOX)to immobilize these enzymes onto the MWCNTs/Pt electrode surfaces. After the GOX was immobilized onto the MWCNTs/Pt electrode, a well-defined catalytic oxidation current was increased from ca. -0.45 V (vs. Ag/AgCl/sat'd KCl), which was close to the redox potential of flavin adenine dinucleotide (FAD)as a prosthetic group of GOX under physiological pH values. Also, fructose dehydrogenase (FDH)immobilized MWCNTs/Pt electrode showed a well-defined catalytic oxidation current based on FDH was observed from ca. -0.15 V (vs. Ag/AgCl/sat'd KCl), which was close to the redox potential of heme c as a prosthetic group of FDH. From an analysis of a plot of the catalytic current vs. substrate, the calibration range for the fructose concentration was up to ca. 40μmol dm^<-3>, and the apparent Michaelis-Menten constant was evaluated to be 11 (±)mmol dm^<-3>Further investigations concerning both the interactions between enzymes and MWCNTs and direct heterogeneous electron transfer reactions are underway to develop sensor applications and glucose-oxygen biofuel cells.