Enhancement of Microbial Function for a Sulfur Redox Cycle Action in a Sewage Treatment Process Combining an UASB and an Aerobic Filter Reactor

本研究的目的是建立一种在低温条件下由微生物的硫-氧化还原循环作用激活的废水处理新工艺。这一作用是由硫酸盐还原菌（SRB）和硫氧化菌（SOB）完成的。反应器系统由UASB反应器作为前处理，DHS反应器作为后处理。反应器运行结果表明，在HRT时间为12hr， UASB反应器温度为8℃的条件下，整个工艺流程的CODcr去除率达到90%以上，最终出水CODcr去除率小于35mg /L （bod去除率小于20mg /L）。受温度产甲烷活性（MPA）和硫酸盐还原活性（SRA）的影响，在25℃条件下，乙酸和H2/CO2作为试验底物的SRA大于MPA。MPA在15℃时几乎没有，SRA在5℃时有一定的变化。提出的硫-氧化还原工艺是一种适用于低温条件下低强度废水处理的工艺。该组合系统还应用于除冰液废水的低温处理。在工作温度为8℃、HRT为12h的条件下，在最终出水CODcr低于30mg /L的情况下，整个工艺的CODcr去除率达到90%以上。在UASB污泥中，Acetobacterium sp.和Desulfobulbus sp.为优势菌种，相对于dapi染色的总细胞的相对丰度分别为10%和14%。

The objective of this research is to make a novel wastewater treatment process activated by a sulfur-redox cycle action of microbe in low temperature conditions. This action is carried out by sulfate-reducing bacteria (SRB) and sulfur-oxidizing bacteria (SOB). The reactor system was comprising of an UASB reactor as pre-treatment and an aerobic downflow hanging sponge (DHS) reactor as post-treatment. As the results of reactor operation, the whole process achieved that over 90% of CODcr removal efficiency, less than 35mg-CODcr/L (less than 20mg-BOD/L) of final effluent, at 12hr of HRT and at 8℃ of UASB reactor temperature. As the result of the effect of temperature methane-producing activity (MPA) and sulfate-reducing activity (SRA) of sludge retained in the UASB reactor, below the 25℃ condition, SRA was greater than MPA from acetate and H2/CO2 as test substrate. And that MPA was all but destitute at 15℃, SRA was certain despite at 5℃. The proposed sulfur-redox action process was considered as an applicable process for low strength wastewater treatment in low temperature conditions.The combined system was also applied for low temperature treatment of deicing fluid wastewater. The whole process achieved over 90% of CODcr removal efficiency with less than 30mg-CODcr/L in the final effluent, at an operating temperature of 8 -C and at an HRT of 12h. In UASB sludge, Acetobacterium sp. and Desulfobulbus sp. were found to be predominant species as the relative abundance to DAPI-stained total cells was 10% and 14%, respectively.