Development of versatile graft polymerization using high temperature decomposing azo initiator having vinyl groups

一种可聚合偶氮引发剂，2，2 ′-偶氮双[N-（2-丙烯基）-2-甲基丙酰胺]（APMPA），具有两个乙烯基和一个偶氮基作为官能团。为了开发APMPA接枝聚合的新方法，研究了APMPA自由基聚合动力学和自制大分子偶氮引发剂的接枝聚合反应。为了扩大该接枝聚合方法的通用性，还进行了APMPA的机械力化学反应试验，由于APMPA的自循环反应，APMPA的均聚不能进行。研究了APMPA与苯乙烯、甲基丙烯酸甲酯、醋酸乙烯酯在其它偶氮引发剂存在下的共聚合反应。采用自制的大分子偶氮引发剂，分别对苯乙烯、甲基丙烯酸甲酯和两亲性大分子单体进行了接枝聚合反应，采用悬浮聚合法制备了季戊四醇三丙烯酸酯、乙二醇二甲基丙烯酸酯和APMPA的粒子，分别对甲基丙烯酸缩水甘油酯（G ...更多信息 MA），并制备具有接枝环氧基团的聚合物颗粒。在其表面上具有接枝环氧基团的聚合物颗粒适合于固定小鼠成纤维细胞系MS-5。在聚合物颗粒存在下培养5天，形成颗粒之间的交联细胞膜以及细胞和颗粒的三维网络。细胞在聚合物颗粒上的粘附（20 min）比在培养皿上的粘附（40 min）快。从固定化MS-5细胞的显微照片来看，HL-60细胞被夹在固定化MS-5细胞之间。在使用APMPA的机械化学反应的情况下，在球磨机中用APMPA处理炭黑纳米颗粒。处理后，APMPA与炭黑纳米颗粒的表面结合。以APMPA共轭炭黑纳米粒子为原料，制备了聚苯乙烯包覆炭黑纳米粒子。少

A polymerizable azo initiator, 2,2'-Azobis[N- (2-propenyl) -2-methylpropionamide](APMPA),has both two vinyl groups and one azo group as functional groups. To develop a new graft-polymerization method using APMPA, radical polymerization kinetics of APMPA and graft-polymerization using produced macromolecular azo initiator were studied. To expand general versatility of this graft polymerization method, mechanochemical reaction using APMPA was also tested.Homo-polymerization of APMPA could not proceed because of APMPA self-circulation reaction. Copolymerization of APMPA with styrene, methyl methacrylate, and vinyl acetate using other azo initiator were progressed. Using produced macromolecular azo initiator, graft-polymerization of styrene, methyl methacrylate or amphiphilic macromonomers were proceeded.Using particles which were produced by suspension polymerization of pentaerythritol triacrylate, ethylene glycol dimethacrylate, and APMPA, graft polymerization of glycidyl methacrylate (G … More MA) was proceeded and polymer particles having the grafted epoxy group were produced. Polymer particles having grafted epoxy group on their surfaces were suitable for immobilization of murine fibroblast cell line MS-5. For 5 days cultivation in the presence of polymer particles, cross-linked cell films between particles and three-dimensional networks of cell and particle were formed. Cell adhesion to polymer particle (20 min) was faster than that to cultivation dish (40 min).To evaluate the hematopoietic support of immobilized MS-5 cells, human myelocytic progenitor cell line HL-60 was co-cultured. From the microphotograph of immobilized MS-5 cells, HL-60 cells were trapped between the immobilized MS-5 cells.In the case of the mechanochemical reaction using APMPA, carbon black nano particles was treated with APMPA in ball mill.After the treatment,APMPA conjugated with the surface of the carbon black nano particle. Using APMPA conjugated carbon black nano particles, poly(styrene)covered carbon black nano particle was produced. Less