Discovery of Food Microorganism : Fast and Effective Degradation of Intestinal Lipoperoxide and Hydrogen peroxide

本研究的目的是发现益生菌乳酸菌作为一种能够分解强烈的过氧化脂质和过氧化氢的功能性食品，并加速其在肠道中形成的降解能力。2005年，我们开发了一种分离具有过氧化脂质降解酶的乳酸菌的分离培养基。此外，我们还筛选出了对过氧化氢具有较强降解能力的潜在菌株。2006年，我们从分离的乳酸菌中筛选出有用的菌株，评价了过氧化氢降解机制的降解能力。经过乳酸菌的基本鉴定和分组(乳酸菌手册，浅仓书店)，我们筛选出了00株可能适用于功能食品的菌株，并从分离的菌株中排除了潜在的致病菌株。通过16sDNA序列测定、分类和Phen…分析，从10株高过氧化脂质分解菌中鉴定出10株更典型的分析。鉴定的结果为植物乳杆菌和戊糖链球菌科。为了测定过氧化氢和脂质过氧化的降解活性，我们制备了乳杆菌标准GYP培养基，在37℃进行好氧培养，然后收获。以亚油酸为过氧化脂质，过氧化异丙苯为模型化合物测定其活性。此外，还使用过氧化氢作为亲水性底物。在分离的菌株中，植物乳杆菌Pan1-2对过氧化氢的降解活性最高，戊糖小球菌BE-I对过氧化氢的降解活性最高。戊糖小球菌属BE-I能清除过氧化异丙苯和过氧化氢，在过氧化丁酯存在下，植物乳杆菌PANL-2与戊糖小球菌属BE-I的反应产物显示，静息细胞菌株对过氧化丁基还原为丁醇的能力相同。结果表明，该菌株具有2电子还原的过氧化作用。较少

The objectives of this research are to discover probiotic lactic acid bacteria as a functional food product enabling strong lipoperoxide and hydrogen peroxide breakdown, and to accelerate its degrading capability being formed in the intestine.In 2005, we developed an isolation medium separated off lactic acid bacteria that has lipoperoxide degradative enzyme. In addition, we isolated potential strains that possess strong degrading capability of hydrogen peroxide. In 2006 identifying useful strains from isolated lactic acid bacteria, we evaluated degrading capability of peroxide-decomposition mechanism.After basic identification and grouping of lactic acid bacteria (lactic acid bacteria Manual, Asakura Bookshop), we screened a total ofl00 strains potentially applicable for functional food product and excluded potential pathogenic strains from isolated strains. 10 out 100 strains performing high lipoperoxide decomposition were full-identified through 16sDNA Sequence and Taxonomy and Phen … More otypic Analysis. As a result of the identification, they are classified as families of Lactobacillus plantarum and Pediococcus pentosaceus.For measuring degradation activity of lipoperoxide and hydrogen peroxide, we prepared for Lactobacillus Standard GYP medium, and conditioned with aerobic culture at 37□ and then harvested. We utilized linoleic acid as a lipid peroxide and cumene peroxide as a model compound to measure its activity. In addition, hydrogen peroxide was used as a hydrophilic substrate. Among isolated strains, Lactobacillus plantarum Pan1-2 performed the highest degrading activity of lipoperoxide and Pediococcus pentosaceus Be-I performed the highest one of hydrogen peroxide. Pediococcus pentosaceus Be-I can scavenge both cumene peroxide and hydrogen peroxide.In presence of butyl peroxide for identifying reaction product of L. plantarum Panl-2 and P pentosaceus Be-I, the strains of resting cell showed equal in reduction of butyl peroxide to butanol. As a result of that, it is suggested that the strains have 2-electron reduction of peroxide. Less