Development of Pro-biotic Lactic Acid Bacteria Scavenging Environmental Hydroperoxides

开发清除环境氢过氧化物的益生乳酸菌

• 批准号: 21580101
• 负责人: NIIMURA Youichi
• 金额: $ 3万
• 依托单位: Tokyo University of Agriculture
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580101/
• 关键词: 乳酸菌; プロバイオティックス; 過酸化脂質; 過酸化脂質分解; 過酸化水素; 過酸化水素分解; Mg-catalase; NADH peroxidase

In order to get lactic acid bacteria scavenging environmental lipid peroxide, we successfully isolated Lactobacillus plantarum P2 strain and purified two lipid peroxide reductases from the bacteria. The enzymes catalyze the reduction of lipid peroxide to its alcohol derivative with NAD(P) H as the preferred electron donor. The gene of the enzyme was cloned and overexpressed in E. coli. The recombinant enzyme has been purified to homogeneity and analyzed to get enzymatic parameters.

为了获得能够清除环境脂质过氧化物的乳酸菌，我们成功分离了植物乳杆菌P2菌株，并从该菌中纯化了两种脂质过氧化物还原酶。这些酶以 NAD(P) H 作为首选电子供体，催化脂质过氧化物还原为其醇衍生物。该酶的基因被克隆并在大肠杆菌中过表达。重组酶已被纯化至均质并进行分析以获得酶学参数。

项目成果

Lactobacillus ozensis sp. nov., a novel lactic acid bacteria isolated from mountain flowers in Oze National Park

奥泽乳杆菌 sp.

• 发表时间: 2011
• 期刊: It. J. Syst. Evol.Microbiol
• 作者: Kawasaki S;Kurosawa K;Miyazaki M;Sakamoto M;Ohkuma M;Y. Niimura
• 通讯作者: Y. Niimura

O2 and Reactive Oxygen Species Detoxification Complex, Composed of O2-Responsive NADH:Rubredoxin Oxidoreductase-Flavoprotein A2-Desulfoferrodoxin Operon Enzymes, Rubperoxin, and Rubredoxin, in Clostridium acetobutylicum

• DOI: 10.1128/aem.01425-08
• 发表时间: 2009-02-15
• 期刊: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
• 影响因子: 4.4
• 作者: Kawasaki, Shinji;Sakai, Yu;Niimura, Youichi
• 通讯作者: Niimura, Youichi

Taxonomical and physiological comparisons of the three species of the genus Amphibacillus.

两栖杆菌属三个物种的分类学和生理学比较。

• 发表时间: 2009
• 期刊: J Gen Appl Microbiol. 55
• 作者: Arai T;Yanahashi S;Sato J;Sato T;Ishikawa M;Koizumi Y;Kawasaki S;Niimura Y;Nakagawa J.
• 通讯作者: Nakagawa J.

Chlorella vulgaris aldehyde reductase is capable of functioning as ferric reductase and of driving the Fenton react ion in the presence of free flavin

小球藻醛还原酶能够起到三价铁还原酶的作用，并在游离黄素存在的情况下驱动芬顿反应

• 发表时间: 2010
• 期刊: Bioscience Biotechnology and Biochemistry
• 影响因子: 1.6
• 作者: J. Sato;K. Takeda;R. Nishiyama;K. Fusayama;T. Arai;T. Sato;T. Watanabe;A. Abe;J. Nakagawa;S. Kawasaki;Y. Niimura
• 通讯作者: Y. Niimura

Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family

• DOI: 10.1111/j.1742-4658.2009.07184.x
• 发表时间: 2009-09
• 期刊: The FEBS Journal
• 作者: N. Shibata;Y. Ueda;Daisuke Takeuchi;Yoshihiro Haruyama;S. Kojima;Junichi Sato;Y. Niimura;M. Kitamura;Y. Higuchi