Discovery of Food Microorganism : Fast and Effective Degradation of Intestinal Lipoperoxide

本研究的目的是：1)发现益生乳酸菌作为一种功能性食品，能够强分解脂过氧化物；2)加速其在肠道内形成的降解能力。2004年，我们开发了一种分离乳酸菌的分离培养基，该培养基含有脂过氧化物降解酶。此外，我们还分离出具有较强过氧化氢降解能力的潜在菌株。2005年，我们从分离的乳酸菌中鉴定出有用的菌株，评估了过氧化氢分解机制的降解能力。标准鉴定和分组：对乳酸菌进行基本鉴定和分组（《乳酸菌手册》，Asakura书店）后，筛选出48株可能适用于功能食品的菌株，并从分离菌株中排除潜在致病菌株。48株高脂过氧化物分解菌株中有4株经16sDNA序列和分类鉴定，并进行表型分析。作为鉴定的结果，它们被归类为植物乳杆菌的一个科。脂过氧化物分解的评价：为了测量脂过氧化物的降解活性，我们在缺乏脂过氧化物和二价铁的条件下制备了乳酸杆菌标准GYP培养基，并在37？然后收获。以亚油酸为过氧化脂类化合物，过氧化异丙烯为模型化合物，测定其活性。此外，过氧化氢被用作亲水性底物。在分离的菌株中，植物乳杆菌Pan1-2对过氧化物的降解活性最高。这种独特的菌株完全降解过氧化苯，同时对作为亲水底物的过氧化氢表现出大约一半的降解活性。静息细胞过氧化降解机制：在植物l.p antarum Pan1-2反应产物过氧化丁基存在的情况下，菌株对过氧化丁基还原为丁醇的能力相等。结果表明，该菌株具有过氧化氢的2电子还原作用。少

The objectives of this research are to 1) discover probiotic lactic acid bacteria as a functional food product enabling strong lipoperoxide breakdown, and 2) to accelerate its degrading capability being formed in the intestine.In 2004, we developed an isolation medium separated off lactic acid bacteria that has lipoperoxide degradative enzyme. In addition, we isolated potential strains that possess strong degrading capability of peroxide. In 2005 identifying useful strains from isolated lactic acid bacteria, we evaluated degrading capability of peroxide-decomposition mechanism.1. Standard Identification and Grouping : After basic identification and grouping of lactic acid bacteria (lactic acid bacteria Manual, Asakura Bookshop), we screened 48 strains potentially applicable for functional food product and excluded potential pathogenic strains from isolated strains. 4 out 48 strains performing high lipoperoxide decomposition were full-identified through 16sDNA Sequence and Taxonomy and … More Phenotypic Analysis. As a result of the identification, they are classified as a family of Lactobacillus plantarum.2. Evaluation of Lipoperoxide Decomposition : For measuring degradation activity of lipoperoxide, we prepared for Lactobacillus Standard GYP medium in lack of lipid peroxide and divalent iron, and conditioned with aerobic culture at 37? and then harvested. We utilized linoleic acid as a lipid peroxide and cumene peroxide as a model compound to measure its activity. In addition, hydrogen peroxide was used as a hydrophilic substrate. Among isolated strains, Lactobacillus plantarum Pan1-2 performed the highest degrading activity of lipoperoxide. This unique strain fully degrades cumene peroxide, while showing approximately a half degrading activity for hydrogen peroxide as a hydrophilic substrate.3. Peroxide Degradation Mechanism of Resting Cell : In presence of butyl peroxide for identifying reaction product of L.plantarum Pan1-2, the strain showed equal in reduction of butyl peroxide to butanol. As a result of that, it is suggested that the strain has 2-electron reduction of peroxide. Less