Gene Transfer of Human Extracellular Superoxide Dismutase Prevents Monocrotaline-Induced Pulmonary Hypertension in Rats

在本研究中，我们研究了气管内转移人细胞外超氧化物歧化酶(EcSOD)基因是否能改善野百合碱(MCT)诱导的大鼠PAH。同时气管内注射β半乳糖苷酶载体(mct组)或人ECSOD腺病毒(AdECSODGal组)或人ECSOD腺病毒(βGal组)。注射MCT 28d后，AdECSOD组右室收缩压、右室/左室加室间隔重量比显著降低。作为这些作用的机制之一，AdECSOD组肺组织中的8-异前列腺素显著减少。肺组织ECSOD过表达可减轻MCT诱导的大鼠PAH。

In the present study, we examined whether intratracheal gene transfer of human extracellular superoxide dismutase (ECSOD) could ameliorate monocrotaline (MCT)-induced PAH in rats. MCT-injected rats were concomitantly intratracheally administered vehicle (MCT group), an adenovirus expressing β-galactosidase (Adβgal group), or human ECSOD (AdECSOD group). Twenty-eight days after the MCT injection, right ventricular systolic pressure and the weight ratio of the right ventricle to the left ventricle plus septum were significantly lower in the AdECSOD group. As the mechanism of these effects, 8-isoprostane in lung tissue was significantly reduced in the AdECSOD group. ECSOD overexpression to the lung ameliorated MCT-induced PAH in rats.