Research on hypoglycemia caused by inhibition of orexin expression

肉碱缺乏症患者发生低血糖的机制尚不清楚。以全身性肉碱缺乏症小鼠(JVS小鼠)为研究对象，以断奶期(产后1428天)为研究对象，对此机制进行了研究。正常小鼠的存活率为100%，且没有观察到低血糖。JVS小鼠第14天存活率为77%，第28天存活率为28%。从第21天开始，出现低血糖。到第17天，胃乳糖几乎完全消失，第14天到第28天几乎检测不到纤维素。在第14天，JVS小鼠和正常RAKE小鼠下丘脑组织中食欲素基因的表达没有差异，但在第21天和第28天，JVS小鼠下丘脑组织中食欲素基因的表达受到抑制。当JVS Mire饲喂肉碱丰富的饲料时，下丘脑食欲素mRNA表达的抑制被消除，在第28天，胃中检测到乳糖和纤维素，而没有低血糖。结论：在断奶期间抑制下丘脑食欲素的表达可能参与了JVS小鼠明显的厌食症，最终导致低血糖死亡。

The mechanism of onset of hypoglycemia in patients with carnitine deficiency has yet to be determined. Using mice with systemic carnitine deficiency (JVS mice), we examined this mechanism, focusing on the weaning period (days 1428 postpartum).For normal mice, the survival rate was 100%, and no hypoglycemia was observed at all. Gastric lactose began to decrease on day 17, and callulose increased sharply in amount thereafter.For JVS mice, the survival rate was 77% on day 14 and 28% on day 28. From day 21 on, hypoglycemia was noted. Gastric lactose had disappeared almost completely by day 17, and cellulose was almost undetectable from days 14 to 28. Expression of orexin mRNA in the hypothalamus did not differ between JVS and normal rake on day 14, but was suppressed in JVS mice on days 21 and 28. When JVS mire were fed a carnitine-rich diet, suppression of expression of orexin mRNA in hypothalamus was eliminated, and on day 28 lactose and cellulose were detected in the stomach without hypoglycemia.In conclusion, the suppression of the expression of orexin in the hypothalamus during the weaning period may be involved in the marked anorexia in JVS mice, which eventually leads to death from hypoglycemia.