Mollecular histopathological study for human papillomavirus type-pecific cytopathic effect

虽然现在人们认为人乳头瘤病毒（HPV）类型和特定的细胞病变（HPV类型特异性细胞病变效应：CPE）之间存在相关性，但CPE的真正机制仍然未知。也没有关于HPV靶细胞的证据。在我们以前的一系列研究中，我们在新的临床类型的病毒疣中发现了HPV 60，63，65，88和95，其中特别是胞浆内包涵体被发现类型特异性。现在公认的是，γ-乳头瘤病毒属由HPV 4、48、50、60、65、88和95组成，而μ属由HPV 1和63组成。这些属的一个特点是以基因型特异的方式产生各种胞质内包涵体（ICBs）。在本研究中，它已被揭示，异质性ICBs，以及它们与特定类型的HPV的关联，将提供一个有用的模型，研究角质形成细胞和HPV之间的相互作用，特别是在E4基因表达的功能方面。除了HPV类型特异性ICB之外，HPV 4/60/65相关色素疣涉及HPV类型特异性黑素生成激活HPV 60提供了HPV相关表皮样囊肿的新临床实体;报告的HPV 1和HPV 63在单个细胞内的双重感染仅与HPV 63相关ICB有关，这提出了病毒之间可能干扰的重要问题：HPV 63诱导的点状疣最近被描述为表皮干细胞的一个很好的线索，其确切的定位尚不清楚;在患者中观察到的HPV 1诱导的增生表明HPV感染不仅诱导可见的病变，而且还激活其邻近的上皮。根据我们的研究结果，我们认为γ-和μ-乳头瘤病毒属在病毒学和人类病理学中提出了需要解决的重要问题

Although it is now accepted that there are correlations between human papillomavirus (HPV) types and specific cytopathic changes (HPV-type-specific cytopathic effect: CPE), the true mechanism of the CPE is still unknown. There are as yet little evidences for the target cells of HPVs, either. In a series of previous studies of our own, we found out HPV 60, 63, 65, 88 and 95 in new clinical types of viral warts, in which particular intracytoplasmic inclusion bodies were seen type-specifically. It is now accepted that genus gamma-papillomavirus consists of HPV 4, 48, 50, 60, 65, 88 and 95, while genus mu HPV 1 and 63. A characteristic of these genera is producing various intracytoplasmic inclusion bodies (ICBs) in genotype-specific manner. In the present study, it has been revealed that the heterogeneous ICBs, and their association with specific types of HPV, will provide a useful model for studying the interaction between keratinocytes and HPVs, especially in the functional aspect of E4 gene expression. Aside from the HPV type-specific ICBs, HPV 4/60/65-associated pigmented warts implicate HPV type-specific activation of melanogenesis HPV 60 provides a new clinical entity of HPV-associated epidermoid cysts; a reported double infection with HPV 1 and HPV 63 within a single cell with only HPV 63-associated ICB poses an important problem of a possible interference between the viruses: HPV 63-induced punctuate wart has recently been described as an excellent clue to epidermal stem cells whose exact localization is not yet clear; and HPV 1-induced hypertrichosis observed in a patient suggests that HPV infection not only induce visible lesions but also activate its neighboring epithelium. On the basis of our results, we suggest that the genus gamma- and mu-papiUomaviruses pose important problems to be resolved in virology and human pathology